CF500745

antibody from Invitrogen Antibodies

Targeting: AKR1A1 ALR, DD3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

CF500745

Provider

Invitrogen Antibodies

Product name

AKR1A1 Monoclonal Antibody (OTI6E3), TrueMAB™

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OTI6E3

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKR1A1 (RC200302, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-AKR1A. Positive lysates LY401826 (100 µg) and LC401826 (20 µg) can be purchased separately from OriGene.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKR1A1 (RC200302, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-AKR1A. Positive lysates LY401826 (100 µg) and LC401826 (20 µg) can be purchased separately from OriGene.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Anti-AKR1A1 mouse monoclonal antibody (TA500745) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AKR1A1(RC200302).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of HepG2 cells using anti-AKR1A1 mouse monoclonal antibody (TA500745).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-AKR1A1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500745, Dilution 1:50)

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells transfected with either pCMV6-ENTRY AKR1A1(RC200302)(Red) or empty vector control plasmid (Blue) were immunostained with anti-AKR1A1 mouse monoclonal(TA500745), and then analyzed by flow cytometry.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of Hela cells, using anti-AKR1A1 antibody (TA500745), (Red) compared to a nonspecific negative control antibody (TA50011)(Blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells transfected with either pCMV6-ENTRY AKR1A1(RC200302)(Red) or empty vector control plasmid (Blue) were immunostained with anti-AKR1A1 mouse monoclonal(TA500745), and then analyzed by flow cytometry.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of Jurkat cells, using anti-AKR1A1 antibody (TA500745), (Red) compared to a nonspecific negative control antibody (TA50011)(Blue).