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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
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- Product number
- PA5-18073 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AKR1A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, canine, mouse, porcine and rat based on sequence homology.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of Human Liver lysate using Product # PA5-18073 at a concentration of 0.1 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of HEK293 overexpressing AKR1A1 using Product # PA5-18073, mock transfection as a control in first lane.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of HEK293 overexpressing AKR1A1 using Product # PA5-18073, mock transfection as a control in first lane.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin embedded of Human Kidney using Product # PA5-18073 at a concentration of 2.5 µg/mL. The tissue was processed by steamed antigen retrieval with citrate buffer pH 6 and stained with AP.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of AKR1A1 in Human Kidney using a AKR1A1 monoclonal antibody (Product #PA5-18073) at 5 µg/mL. The Human Kidney tissue section was paraffin embeded and detected using Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
