PAB10207

antibody from Abnova Corporation

Targeting: FBXO5 EMI1, FBX5, Fbxo31

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB10207

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB10207, RRID:AB_1674716

Product name

FBXO5 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of FBXO5.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Plk1 regulates activation of the anaphase promoting complex by phosphorylating and triggering SCFbetaTrCP-dependent destruction of the APC Inhibitor Emi1.

Hansen DV, Loktev AV, Ban KH, Jackson PK
Molecular biology of the cell 2004 Dec;15(12):5623-34

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E2F-dependent accumulation of hEmi1 regulates S phase entry by inhibiting APC(Cdh1).

Hsu JY, Reimann JD, Sørensen CS, Lukas J, Jackson PK
Nature cell biology 2002 May;4(5):358-66

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Emi1 is required for cytostatic factor arrest in vertebrate eggs.

Reimann JD, Jackson PK
Nature 2002 Apr 25;416(6883):850-4

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using FBXO5 polyclonal antibody (Cat # PAB10207) shows detection of a major band corresponding to FBXO5 protein in a human HeLa whole cell lysate (Lane 1 arrowhead).The identity of cross-reactive minor bands at ~72 KDa and 150 KDa is unknown.Specific band staining is blocked when the antibody is pre-incubated with the immunizing peptide (Lane 2).Approximately 33 ug of lysate was loaded per lane onto a 4-20% gradient gel followed by transfer to nitrocellulose.The membrane was blocked using BLOTTO.Primary antibody was used at a 1:500 dilution in BLOTTO.The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated affinity purified Goat-anti-Rabbit IgG [H&L] MX10 (800 nm channel, green).Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (Lane M, 700 nm channel, red).

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemistry of FBXO5 polyclonal antibody (Cat # PAB10207) shows strong cytoplasmic and membranous staining of bile duct cells in human liver tissue.Tissue was formalin-fixed and paraffin embedded.Brown color indicates presence of protein, blue color shows cell nuclei.Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)