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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA1-30319 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RYK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Detects a band of approximately 67.5 kDa (predicted molecular weight: 72 kDa).
- Concentration
- 0.40 mg/mL
Submitted references Wnt signaling contributes to withdrawal symptoms from opioid receptor activation induced by morphine exposure or chronic inflammation.
Wu M, Li Z, Liang L, Ma P, Cui D, Chen P, Wu G, Song XJ
Pain 2020 Mar;161(3):532-544
Pain 2020 Mar;161(3):532-544
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of RYK in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the RYK gene (Lane 2) using a RYK polyclonal antibody (Product # PA1-30319).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RYK was performed by loading Jurkat cell lysate. Proteins were transferred to a membrane and probed with a RYK Polyclonal Antibody (Product # PA1-30319).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RYK was performed by loading 20 µg of NIH3T3 cell lysates. Proteins were transferred to a membrane and probed with a RYK Polyclonal Antibody (Product # PA1-30319) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RYK was performed by loading Jurkat cell lysate. Proteins were transferred to a membrane and probed with a RYK Polyclonal Antibody (Product # PA1-30319).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RYK was performed by loading 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the RYK (Lane 2). Proteins were transferred to a membrane and probed with a RYK Polyclonal Antibody (Product # PA1-30319).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of RYK in human breast carcinoma tissue using RYK Polyclonal Antibody (Product # PA1-30319).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
