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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00002827-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00002827-M01, RRID:AB_539001
- Product name
- Mouse Monoclonal GPR3 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. GPR3 - G protein-coupled receptor 3
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GPR3 Antibody (3B4-G3) [H00002827-M01] - Analysis of GPR3 over-expressed 293 cell line, cotransfected with GPR3 Validated Chimera RNAi ( Cat # H00002827-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GPR3 monoclonal antibody (M01), clone 3B4-G3 (Cat # H00002827-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GPR3 Antibody (3B4-G3) [H00002827-M01] - Analysis of GPR3 expression in transfected 293T cell line by GPR3 monoclonal antibody (M01), clone 3B4-G3.Lane 1: GPR3 transfected lysate(35 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GPR3 Antibody (3B4-G3) [H00002827-M01] - GPR3 monoclonal antibody (M01), clone 3B4-G3 Analysis of GPR3 expression in Jurkat.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: GPR3 Antibody (3B4-G3) [H00002827-M01] - Detection limit for recombinant GST tagged GPR3 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GPR3 Antibody (3B4-G3) [H00002827-M01] - Analysis of monoclonal antibody to GPR3 on formalin-fixed paraffin-embedded human transitional cell carcinoma tissue. Antibody concentration 1 ug/ml.
