Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [2]
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Validation data
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- Product number
- PA5-38480 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Phospho-EphB1/EphB2 (Tyr594, Tyr604) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references TAGAP instructs Th17 differentiation by bridging Dectin activation to EPHB2 signaling in innate antifungal response.
Chen J, He R, Sun W, Gao R, Peng Q, Zhu L, Du Y, Ma X, Guo X, Zhang H, Tan C, Wang J, Zhang W, Weng X, Man J, Bauer H, Wang QK, Martin BN, Zhang CJ, Li X, Wang C
Nature communications 2020 Apr 20;11(1):1913
Nature communications 2020 Apr 20;11(1):1913
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-EphB1 pTyr594 + EphB2 pTyr604 in extracts from HepG2 cells using a Phospho-EphB1 pTyr594 + EphB2 pTyr604 polyclonal antibody (Product # PA5-38480).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Phospho-EphB1 pTyr594 + EphB2 pTyr604 in HUVEC cells using a Phospho-EphB1 pTyr594 + EphB2 pTyr604 polyclonal antibody (Product # PA5-38480).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 EPHB2 phosphorylates TAGAP at tyrosine 310. a THP-1 cells were stimulated with heat-killed C. albicans (MOI = 2) for indicated times, followed by western blot analysis for indicated proteins. b HEK293T cells were transfected with indicated plasmids, and cell lysates were immunoprecipitated with anti-HA antibody, followed by immunoblot analysis for indicated proteins. c HEK293T cells were transfected with Flag-Syk WT and KD, and cell lysates were immunoprecipitated with anti-Flag antibody, followed by in vitro kinase assay by incubating with purified His-EPHB2 as substrate. Immunoblot was conducted for the analysis for indicated proteins. d - f HEK293T cells were transfected with indicated plasmids, and cell lysates were immunoprecipitated with anti-HA antibody, followed by immunoblot analysis for indicated proteins. g Schematic presentation of conservativeness of TAGAP Y310 site between different species was shown. h TAGAP-gRNA-infected THP-1 cells were infected with indicated plasmids by lentivirus expressing vectors, and cells were stimulated with heat-killed C. albicans (MOI = 2) for indicated times, followed by western blot analysis of indicated protein expression. i HEK293T cells were transfected with indicated plasmids, and cell lysates were immunoprecipitated with anti-HA antibody, followed by immunoblot analysis for indicated proteins. j Hela cells were transfected with indicated plasmids, and immunofluorescence was done by staining HA and Flag antibodies. Scal
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 8 Vandetanib and Dasatinib attenuate EAE severity. a EAE Clinical score of heterozygous control mice or TAGAP-deficient mice in EAE model was shown ( n = 5). b Hematoxylin and Luxol fast blue staining of transversal sections of lumbar spinal cords from heterozygous control or TAGAP-deficient mice with EAE. Scale bars represent 200 mum. Arrows in the upper panel indicate inflammatory cells infiltration, and arrows in the lower panel indicate demyelination area. c RT and real-time PCR analysis of inflammatory gene expression in spinal cords from EAE mice (n = 5) of indicated genotypes were shown. d Infiltrating cells in the brains of heterozygous control or TAGAP-deficient mice with EAE ( n = 5) were isolated at the peak of the disease (21 days after EAE induction), followed by flow cytometry analysis ( n = 5). e - g HEK293T cells were transfected with indicated plasmids, and 24 h after transfection, cells were treated with Dasatinib e and Vandetanib f at indicated doses for another 24 h, and cell lysates were immunoprecipitated with anti-Flag e , f or HA g antibody, followed by immunoblot analysis for indicated proteins. h , i BMDMs from wild-type mice were pretreated with Dasatinib (0.3 mu m ) or Vandetanib (2 mu m ) for 24 h, and cells were stimulated with Curdlan (100 mug/ml) h or alpha-Mannan (100 mug/ml) i for indicated times, followed by RT and real-time PCR analysis of indicated gene expression. j Clinical score of control and Vandetanib and Dasatinib-treated mice