Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
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Validation data
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- Product number
- ARG54851 - Provider product page
- Provider
- Arigo
- Product name
- anti-EphB1 antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated synthetic peptide around aa. 955-984 (C-terminus) of Human EphB1.
- Description
- Purification with Protein G.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 6 months. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: mouse brain tissue stained with ARG54851 anti-EphB1 antibody.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 35 µg of T47D cell line lysates stained with ARG54851 anti-EphB1 antibody.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 35 µg of A431, Hela, MDA-MB-453 cell line (from left to right) stained with ARG54851 anti-EphB1 antibody at 1:1000 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: EphB1 in gastric cancer tissues stained with ARG54851 anti-EphB1 antibody. (a) EphB1 protein expressed in normal mucosa at the glandular compartment and in a decreasing gradient from the glandular compartment to the foveolar compartment. (b) EphB1 protein focally positively stained in well-differentiated gastric cancer cells. (c) EphB1 protein is focally positive in poorly differentiated gastric cancer cells. (d) Loss of EphB1 expression in gastric cancer cells. (Provided by Jian-dong Wang, Department of Pathology Nanjing Jinling Hospital/Nanjing University School of Medicine).
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: formalin-fixed and paraffin-embedded human cancer tissue stained with ARG54851 anti-EphB1 antibody.