Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AER-021-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EphB1 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 25 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes: - 1,2. Anti-EphB1 (extracellular) Antibody (#AER-021), (1:800).3,4. Anti-EphB1 (extracellular) Antibody , preincubated with EphB1 (extracellular) Blocking Peptide (#BLP-ER021).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of EphB1 in rat PC12 cells - Cell surface detection of EphB1 in live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-EphB1 (extracellular) Antibody (#AER-021), (1:100), followed by goat Anti-rabbit-AlexaFluor-594 (red). B. Live image of the cells. C. Merge of the two images.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of EphB1 in live intact human THP-1 monocytic leukemia cell line: - (black) Cells + goat Anti-rabbit-AlexaFluor-488 secondary Antibody . (green) Cells + Anti-EphB1 (extracellular) Antibody (#AER-021), (1:15) + goat- Anti-rabbit-Alexa-488.