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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MA5-15385 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EphB1 Monoclonal Antibody (5F10A4)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15385 targets EhpB1 in indirect ELISA, IHC and WB applications and shows reactivity with Human samples. The MA5-15385 immunogen is purified recombinant fragment of EphB1 (aa19-133) expressed in E. Coli. . MA5-15385 detects EhpB1 which has a predicted molecular weight of approximately 110kDa.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5F10A4
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Identification of a novel agrin-dependent pathway in cell signaling and adhesion within the erythroid niche.
Anselmo A, Lauranzano E, Soldani C, Ploia C, Angioni R, D'amico G, Sarukhan A, Mazzon C, Viola A
Cell death and differentiation 2016 Aug;23(8):1322-30
Cell death and differentiation 2016 Aug;23(8):1322-30
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EhpB1 using EhpB1 monoclonal antibody (Product # MA5-15385) in MDA-MB-468 (1), MDA-MB-453 (2), MCF-7 (3), T47D (4) and SKBR-3 (5) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and colon cancer (right) showing cytoplasmic localization using EhpB1 monoclonal antibody (Product # MA5-15385) followed with DAB staining
