A301-037A

antibody from Invitrogen Antibodies

Targeting: SCAF8 KIAA1116, RBM16

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A301-037A

Provider

Invitrogen Antibodies

Product name

RBM16 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.20 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse RBM16 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RBM16 antibody A301-037A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B), and used for IP at 3 µg/mg lysate. RBM16 was also immunoprecipitated by rabbit anti-RBM16 antibodies A301-036A and BL4483, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human RBM16 by immunohistochemistry. Sample: FFPE section of human skin basal cell carcinoma. Antibody: Affinity purified rabbit anti- RBM16 (Cat. No. A301-037A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB.