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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-21647 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PCCB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2.
- Concentration
- 0.54 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PCCB Polyclonal Antibody (Product # PA5-21647). Sample (30 µg whole cell lysate). A: HepG2. 7.5% SDS PAGE. PCCB Polyclonal Antibody (Product # PA5-21647) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PCCase beta in methanol-fixed Hep G2 cells using a PCCase beta polyclonal antibody (Product # PA5-21647) at a 1:500 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed Hep G2, using PCCB (Product # PA5-21647) antibody at 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Lung Ad Cancer PC14 xenograft, using PCCB Polyclonal Antibody (Product # PA5-21647) at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PCCB Polyclonal Antibody detects PCCB protein at mitochondria on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. PCCB Polyclonal Antibody (Product # PA5-21647) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
