F54774

antibody from NSJ Bioreagents

Targeting: GADD45A DDIT1, GADD45

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54774

Provider

NSJ Bioreagents

Product name

GADD45A Antibody

Antibody type

Polyclonal

Description

This highly specific GADD45A antibody is suitable for use in Western blot/Flow cytometry/Immunohistochemistry applications with human samples.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the GADD45A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with GADD45A antibody. Expected molecular weight: 14-18 kDa (monomer) and 28-36 kDa (dimer).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human tonsil tissue with GADD45A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human K562 cells with GADD45A antibody; Blue=isotype control, Green= GADD45A antibody.