BS-1360R

antibody from Invitrogen Antibodies

Targeting: GADD45A DDIT1, GADD45

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

BS-1360R

Provider

Invitrogen Antibodies

Product name

GADD45 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

L1 rat brain lysates L2 rat kidney lysates probed with Rabbit Anti-GADD45 Polyclonal Antibody, Unconjugated (bs-1360R) at 1:200 overnight at 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37°C. Predicted band 18 kD. Observed band size: 18 kD.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

L1 rat brain lysates L2 rat kidney lysates probed with Rabbit Anti-GADD45 Polyclonal Antibody, Unconjugated (bs-1360R) at 1:200 overnight at 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37°C. Predicted band 18 kD. Observed band size: 18 kD.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed and paraffin embedded rat heart labeled with Anti-GADD45 Polyclonal Antibody, Unconjugated (bs-1360R) followed by conjugation to the secondary antibody and DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed and paraffin embedded rat brain labeled with Anti-GADD45 Polyclonal Antibody, Unconjugated (bs-1360R) followed by conjugation to the secondary antibody and DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GADD45 Polyclonal Antibody, Unconjugated (bs-1360R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.