Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-49530 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Gng12 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Predicted to react with bovine and mouse based on sequence homology.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.25 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GNG12 in (Lane 1) Hela whole cell lysate (20 µg), (Lane 2) DU145 whole cell lysate (20 µg), (Lane 3) mouse brain lysate (20 µg). Samples were probed with GNG12 Antibody (C-Term) (Product # PA5-49530) at 1:2000 dilution, followed by Secondary Goat Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size : 8 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GNG12 in (Lane 1) Hela whole cell lysate (20 µg), (Lane 2) DU145 whole cell lysate (20 µg), (Lane 3) mouse brain lysate (20 µg). Samples were probed with GNG12 Antibody (C-Term) (Product # PA5-49530) at 1:2000 dilution, followed by Secondary Goat Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size : 8 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GNG12 in paraformaldehyde-fixed, paraffin-embedded human brain tissue. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated using a GNG12 polyclonal antibody (Product # PA5-49530) 1:25 for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.