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- References [6]
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- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Other assay [2]
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- Product number
- MA1-16578 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPE65 Monoclonal Antibody (401.8B11.3D9)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Porcine, Xenopus
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 401.8B11.3D9
- Vial size
- 200 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C or -80°C if preferred
Submitted references Derivation and Characterization of Murine and Amphibian Müller Glia Cell Lines.
Connective tissue growth factor promotes retinal pigment epithelium mesenchymal transition via the PI3K/AKT signaling pathway.
Leucinostatin acts as a co-inducer for heat shock protein 70 in cultured canine retinal pigment epithelial cells.
Transcriptome-Wide Analysis of CXCR5 Deficient Retinal Pigment Epithelial (RPE) Cells Reveals Molecular Signatures of RPE Homeostasis.
Retinal differentiation of human bone marrow-derived stem cells by co-culture with retinal pigment epithelium in vitro.
Cleavage of the retinal pigment epithelium-specific protein RPE65 under oxidative stress.
Gallo RA, Qureshi F, Strong TA, Lang SH, Pino KA, Dvoriantchikova G, Pelaez D
Translational vision science & technology 2022 Apr 1;11(4):4
Translational vision science & technology 2022 Apr 1;11(4):4
Connective tissue growth factor promotes retinal pigment epithelium mesenchymal transition via the PI3K/AKT signaling pathway.
Wang Y, Chang T, Wu T, Ye W, Wang Y, Dou G, Du H, Hui Y, Guo C
Molecular medicine reports 2021 May;23(5)
Molecular medicine reports 2021 May;23(5)
Leucinostatin acts as a co-inducer for heat shock protein 70 in cultured canine retinal pigment epithelial cells.
Lyu Q, Ludwig IS, Kooten PJS, Sijts AJAM, Rutten VPMG, van Eden W, Broere F
Cell stress & chaperones 2020 Mar;25(2):235-243
Cell stress & chaperones 2020 Mar;25(2):235-243
Transcriptome-Wide Analysis of CXCR5 Deficient Retinal Pigment Epithelial (RPE) Cells Reveals Molecular Signatures of RPE Homeostasis.
Saddala MS, Lennikov A, Mukwaya A, Huang H
Biomedicines 2020 Jun 1;8(6)
Biomedicines 2020 Jun 1;8(6)
Retinal differentiation of human bone marrow-derived stem cells by co-culture with retinal pigment epithelium in vitro.
Mathivanan I, Trepp C, Brunold C, Baerlocher G, Enzmann V
Experimental cell research 2015 Apr 10;333(1):11-20
Experimental cell research 2015 Apr 10;333(1):11-20
Cleavage of the retinal pigment epithelium-specific protein RPE65 under oxidative stress.
Lee H, Chung H, Arnouk H, Lamoke F, Hunt RC, Hrushesky WJ, Wood PA, Lee SH, Jahng WJ
International journal of biological macromolecules 2010 Aug 1;47(2):104-8
International journal of biological macromolecules 2010 Aug 1;47(2):104-8
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RPE65 detected in bovine and human samples. Lane 1: bovine RPE membrane, lane 2: recombinant human RPE transfected COS7 cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RPE65 in 20 µg lysate of COS7 cells. Samples were incubated in RPE65 monoclonal antibody (Product # MA1-16578) followed by an alkaline phosphatase conjugated goat-anti Mouse IgG secondary antibody. Recombinant Human RPE65 (Lane 1) and 5 µg of Bovine retinal pigment epithelium membrane fraction (Lane 2).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of RPE65 in ARPE19 cells, a spontaneously arising human retinal pigment epithelia cell line. Samples were incubated in RPE65 monoclonal antibody (Product # MA1-16578) using a dilution of 1:100 dilution in PBS, overnight 4 °C incubation. 10 minutes fixation in 4% PFA, 10 minutes permeabilization in PBS containing 0.2% Triton X-100 (PBS-T), 1 hour blocking in 10% normal goat serum containing 1% BSA in PBS-T.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of RPE65 in cryosection of mouse retina tissue. Frozen samples were incubated in RPE65 monoclonal antibody (Product # MA1-16578).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of RPE65 in cryosection of mouse retina tissue. Frozen samples were incubated in RPE65 monoclonal antibody (Product # MA1-16578).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of RPE65 in formalin-fixed paraffin-embedded human glioblastoma tissue section. Samples were incubated in RPE65 monoclonal antibody (Product # MA1-16578) using a dilution of 1:500. Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1. Effects of CTGF on EMT and the ECM synthesis. (A) Immunohistochemistry of surgically excised human PVR specimens stained for CTGF, fibronectin and collagen III. Original magnification, x400 and x800 (enlargement of boxed area). (B) Immunofluorescence co-staining of PVR membranes for CTGF (red) and RPE65 (green). Original magnification, x400 (C). Relative mRNA expression levels of biomarkers for EMT and the ECM synthesis in ARPE19 cells treated with CTGF (15, 30 and 60 ng/ml), including specific markers of epithelial cells (ZO-1 and E-cadherin), mesenchymal cells (fibronectin, N-cadherin and alpha-SMA) and the ECM (collagen type III). (D) Representative western blot analysis of the expression levels of EMT markers in ARPE19 cells treated with CTGF (15, 30 and 60 ng/ml). (E) Quantification of relative protein expression of ZO-1, E-cadherin and N-cadherin. (F) Representative western blot analysis of the expression levels of EMT and ECM synthesis markers in ARPE19 cells treated with CTGF (15, 30 and 60 ng/ml). (G) Relative protein expression levels of fibronectin, alpha-SMA and collagen type III; n=3. *P
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2. Immunophenotypic characterization of X laevis MG cell line XG69. (A) Phase contrast images and immunofluorescence staining of primary MG and XG69 cell line at passage 20. (B) Expression of MG genes in primary MG and XG69 cells (passages 20-25). The geometric means and geometric standard deviations ( N = 3 independent cultures) are graphed. An unpaired t -test was applied with a P value of
