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- Antibody Data
- Antigen structure
- References [47]
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- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- ABIN151819 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Retinal Pigment Epithelium-Specific Protein 65kDa (RPE65) antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Porcine, Xenopus
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 401-8B11-3D9
- Vial size
- 0.2 mL
- Storage
- Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
- Handling
- Avoid freeze-thaw cycles
Submitted references IGF-1-Mediated Survival from Induced Death of Human Primary Cultured Retinal Pigment Epithelial Cells Is Mediated by an Akt-Dependent Signaling Pathway.
Patient-specific mutations impair BESTROPHIN1's essential role in mediating Ca2+-dependent Cl- currents in human RPE.
Cooperation of Rel family members in regulating Aβ1-40-mediated pro-inflammatory cytokine secretion by retinal pigment epithelial cells.
BMP-induced reprogramming of the neural retina into retinal pigment epithelium requires Wnt signalling.
Course of Sodium Iodate-Induced Retinal Degeneration in Albino and Pigmented Mice.
A novel coculture model of porcine central neuroretina explants and retinal pigment epithelium cells.
Assessment of visual function and retinal structure following acute light exposure in the light sensitive T4R rhodopsin mutant dog.
Protective effects of retinoid x receptors on retina pigment epithelium cells.
Retinal pigment epithelial cell multinucleation in the aging eye - a mechanism to repair damage and maintain homoeostasis.
Hydrogel for Simultaneous Tunable Growth Factor Delivery and Enhanced Viability of Encapsulated Cells in Vitro.
Choroidal Involution Is Associated with a Progressive Degeneration of the Outer Retinal Function in a Model of Retinopathy of Prematurity: Early Role for IL-1β.
The Complement Regulatory Protein CD46 Deficient Mouse Spontaneously Develops Dry-Type Age-Related Macular Degeneration-Like Phenotype.
Induction of ectopic retina-like tissue by transgenic expression of neurogenin.
Adenovirus-mediated delivery of Factor H attenuates complement C3 induced pathology in the murine retina: a potential gene therapy for age-related macular degeneration.
Expression and cellular localization of the Mas receptor in the adult and developing mouse retina.
Bioactive substrates for human retinal pigment epithelial cell growth from elastin-like recombinamers.
Lignin Induces ES Cells to Differentiate into Neuroectodermal Cells through Mediation of the Wnt Signaling Pathway.
Recombinant AAV-mediated BEST1 transfer to the retinal pigment epithelium: analysis of serotype-dependent retinal effects.
TNF-α disrupts morphologic and functional barrier properties of polarized retinal pigment epithelium.
Histology and immunochemistry evaluation of autologous translocation of retinal pigment epithelium-choroid graft in porcine eyes.
Regulation of T-lymphocyte CCL3 and CCL4 production by retinal pigment epithelial cells.
RPE specification in the chick is mediated by surface ectoderm-derived BMP and Wnt signalling.
Retinal astrocytes pretreated with NOD2 and TLR2 ligands activate uveitogenic T cells.
Generation of retinal pigment epithelial cells from human embryonic stem cell-derived spherical neural masses.
ZIP2 and ZIP4 mediate age-related zinc fluxes across the retinal pigment epithelium.
Short-interfering RNAs induce retinal degeneration via TLR3 and IRF3.
Generation of retinal pigment epithelial cells from small molecules and OCT4 reprogrammed human induced pluripotent stem cells.
Equivalence of conventionally-derived and parthenote-derived human embryonic stem cells.
Enhanced retinal pigment epithelium regeneration after injury in MRL/MpJ mice.
Mitochondria impairment correlates with increased sensitivity of aging RPE cells to oxidative stress.
Altered expression of retinal molecular markers in the canine RPE65 model of Leber congenital amaurosis.
A protocol for the culture and differentiation of highly polarized human retinal pigment epithelial cells.
Steroids do not prevent photoreceptor degeneration in the light-exposed T4R rhodopsin mutant dog retina irrespective of AP-1 inhibition.
The in vitro and in vivo behaviour of retinal pigment epithelial cells cultured on ultrathin collagen membranes.
CD133 progenitor cells from the bone marrow contribute to retinal pigment epithelium repair.
Retinal Astrocytes respond to IL-17 differently than Retinal Pigment Epithelial cells.
Induction of retinal pigment epithelium properties in ciliary margin progenitor cells.
Iron regulates L-cystine uptake and glutathione levels in lens epithelial and retinal pigment epithelial cells by its effect on cytosolic aconitase.
Reactivation of uveitogenic T cells by retinal astrocytes derived from experimental autoimmune uveitis-prone B10RIII mice.
Characterization of a spontaneous mouse retinal pigment epithelial cell line B6-RPE07.
Novel organotypic culture model of adult mammalian neurosensory retina in co-culture with retinal pigment epithelium.
Aging enhances pressure-induced arterial superoxide formation.
Human cone photoreceptor dependence on RPE65 isomerase.
Genetics and phenotypes of RPE65 mutations in inherited retinal degeneration.
Rpe65 is necessary for production of 11-cis-vitamin A in the retinal visual cycle.
Molecular characterization of the human gene encoding an abundant 61 kDa protein specific to the retinal pigment epithelium.
Molecular cloning and expression of RPE65, a novel retinal pigment epithelium-specific microsomal protein that is post-transcriptionally regulated in vitro.
Zheng W, Meng Q, Wang H, Yan F, Little PJ, Deng X, Lin S
Molecular neurobiology 2018 Mar;55(3):1915-1927
Molecular neurobiology 2018 Mar;55(3):1915-1927
Patient-specific mutations impair BESTROPHIN1's essential role in mediating Ca2+-dependent Cl- currents in human RPE.
Li Y, Zhang Y, Xu Y, Kittredge A, Ward N, Chen S, Tsang SH, Yang T
eLife 2017 Oct 24;6
eLife 2017 Oct 24;6
Cooperation of Rel family members in regulating Aβ1-40-mediated pro-inflammatory cytokine secretion by retinal pigment epithelial cells.
Sun J, Huang P, Liang J, Li J, Shen M, She X, Feng Y, Luo X, Liu T, Sun X
Cell death & disease 2017 Oct 12;8(10):e3115
Cell death & disease 2017 Oct 12;8(10):e3115
BMP-induced reprogramming of the neural retina into retinal pigment epithelium requires Wnt signalling.
Steinfeld J, Steinfeld I, Bausch A, Coronato N, Hampel ML, Depner H, Layer PG, Vogel-Höpker A
Biology open 2017 Jul 15;6(7):979-992
Biology open 2017 Jul 15;6(7):979-992
Course of Sodium Iodate-Induced Retinal Degeneration in Albino and Pigmented Mice.
Chowers G, Cohen M, Marks-Ohana D, Stika S, Eijzenberg A, Banin E, Obolensky A
Investigative ophthalmology & visual science 2017 Apr 1;58(4):2239-2249
Investigative ophthalmology & visual science 2017 Apr 1;58(4):2239-2249
A novel coculture model of porcine central neuroretina explants and retinal pigment epithelium cells.
Di Lauro S, Rodriguez-Crespo D, Gayoso MJ, Garcia-Gutierrez MT, Pastor JC, Srivastava GK, Fernandez-Bueno I
Molecular vision 2016;22:243-53
Molecular vision 2016;22:243-53
Assessment of visual function and retinal structure following acute light exposure in the light sensitive T4R rhodopsin mutant dog.
Iwabe S, Ying GS, Aguirre GD, Beltran WA
Experimental eye research 2016 May;146:341-53
Experimental eye research 2016 May;146:341-53
Protective effects of retinoid x receptors on retina pigment epithelium cells.
Ayala-Peña VB, Pilotti F, Volonté Y, Rotstein NP, Politi LE, German OL
Biochimica et biophysica acta 2016 Jun;1863(6 Pt A):1134-45
Biochimica et biophysica acta 2016 Jun;1863(6 Pt A):1134-45
Retinal pigment epithelial cell multinucleation in the aging eye - a mechanism to repair damage and maintain homoeostasis.
Chen M, Rajapakse D, Fraczek M, Luo C, Forrester JV, Xu H
Aging cell 2016 Jun;15(3):436-45
Aging cell 2016 Jun;15(3):436-45
Hydrogel for Simultaneous Tunable Growth Factor Delivery and Enhanced Viability of Encapsulated Cells in Vitro.
Parker J, Mitrousis N, Shoichet MS
Biomacromolecules 2016 Feb 8;17(2):476-84
Biomacromolecules 2016 Feb 8;17(2):476-84
Choroidal Involution Is Associated with a Progressive Degeneration of the Outer Retinal Function in a Model of Retinopathy of Prematurity: Early Role for IL-1β.
Zhou TE, Rivera JC, Bhosle VK, Lahaie I, Shao Z, Tahiri H, Zhu T, Polosa A, Dorfman A, Beaudry-Richard A, Costantino S, Lodygensky GA, Lachapelle P, Chemtob S
The American journal of pathology 2016 Dec;186(12):3100-3116
The American journal of pathology 2016 Dec;186(12):3100-3116
The Complement Regulatory Protein CD46 Deficient Mouse Spontaneously Develops Dry-Type Age-Related Macular Degeneration-Like Phenotype.
Lyzogubov VV, Bora PS, Wu X, Horn LE, de Roque R, Rudolf XV, Atkinson JP, Bora NS
The American journal of pathology 2016 Aug;186(8):2088-2104
The American journal of pathology 2016 Aug;186(8):2088-2104
Induction of ectopic retina-like tissue by transgenic expression of neurogenin.
Yan RT, He L, Zhan W, Wang SZ
PloS one 2015;10(1):e0116171
PloS one 2015;10(1):e0116171
Adenovirus-mediated delivery of Factor H attenuates complement C3 induced pathology in the murine retina: a potential gene therapy for age-related macular degeneration.
Cashman SM, Gracias J, Adhi M, Kumar-Singh R
The journal of gene medicine 2015 Oct-Dec;17(10-12):229-43
The journal of gene medicine 2015 Oct-Dec;17(10-12):229-43
Expression and cellular localization of the Mas receptor in the adult and developing mouse retina.
Prasad T, Verma A, Li Q
Molecular vision 2014;20:1443-55
Molecular vision 2014;20:1443-55
Bioactive substrates for human retinal pigment epithelial cell growth from elastin-like recombinamers.
Singh AK, Srivastava GK, Martín L, Alonso M, Pastor JC
Journal of biomedical materials research. Part A 2014 Mar;102(3):639-46
Journal of biomedical materials research. Part A 2014 Mar;102(3):639-46
Lignin Induces ES Cells to Differentiate into Neuroectodermal Cells through Mediation of the Wnt Signaling Pathway.
Inoue Y, Hasegawa S, Yamada T, Date Y, Mizutani H, Nakata S, Akamatsu H
PloS one 2013;8(6):e66376
PloS one 2013;8(6):e66376
Recombinant AAV-mediated BEST1 transfer to the retinal pigment epithelium: analysis of serotype-dependent retinal effects.
Guziewicz KE, Zangerl B, Komáromy AM, Iwabe S, Chiodo VA, Boye SL, Hauswirth WW, Beltran WA, Aguirre GD
PloS one 2013;8(10):e75666
PloS one 2013;8(10):e75666
TNF-α disrupts morphologic and functional barrier properties of polarized retinal pigment epithelium.
Shirasawa M, Sonoda S, Terasaki H, Arimura N, Otsuka H, Yamashita T, Uchino E, Hisatomi T, Ishibashi T, Sakamoto T
Experimental eye research 2013 May;110:59-69
Experimental eye research 2013 May;110:59-69
Histology and immunochemistry evaluation of autologous translocation of retinal pigment epithelium-choroid graft in porcine eyes.
Fernandez-Bueno I, Rodriguez de la Rua E, Hileeto D, Parrado ML, Regueiro-Purriños M, Sala-Puigdollers A, Srivastava GK, Gonzalo-Orden JM, Pastor JC
Acta ophthalmologica 2013 Mar;91(2):e125-32
Acta ophthalmologica 2013 Mar;91(2):e125-32
Regulation of T-lymphocyte CCL3 and CCL4 production by retinal pigment epithelial cells.
Wallace CA, Moir G, Malone DF, Duncan L, Devarajan G, Crane IJ
Investigative ophthalmology & visual science 2013 Jan 28;54(1):722-30
Investigative ophthalmology & visual science 2013 Jan 28;54(1):722-30
RPE specification in the chick is mediated by surface ectoderm-derived BMP and Wnt signalling.
Steinfeld J, Steinfeld I, Coronato N, Hampel ML, Layer PG, Araki M, Vogel-Höpker A
Development (Cambridge, England) 2013 Dec;140(24):4959-69
Development (Cambridge, England) 2013 Dec;140(24):4959-69
Retinal astrocytes pretreated with NOD2 and TLR2 ligands activate uveitogenic T cells.
Jiang G, Sun D, Kaplan HJ, Shao H
PloS one 2012;7(7):e40510
PloS one 2012;7(7):e40510
Generation of retinal pigment epithelial cells from human embryonic stem cell-derived spherical neural masses.
Cho MS, Kim SJ, Ku SY, Park JH, Lee H, Yoo DH, Park UC, Song SA, Choi YM, Yu HG
Stem cell research 2012 Sep;9(2):101-9
Stem cell research 2012 Sep;9(2):101-9
ZIP2 and ZIP4 mediate age-related zinc fluxes across the retinal pigment epithelium.
Leung KW, Gvritishvili A, Liu Y, Tombran-Tink J
Journal of molecular neuroscience : MN 2012 Jan;46(1):122-37
Journal of molecular neuroscience : MN 2012 Jan;46(1):122-37
Short-interfering RNAs induce retinal degeneration via TLR3 and IRF3.
Kleinman ME, Kaneko H, Cho WG, Dridi S, Fowler BJ, Blandford AD, Albuquerque RJ, Hirano Y, Terasaki H, Kondo M, Fujita T, Ambati BK, Tarallo V, Gelfand BD, Bogdanovich S, Baffi JZ, Ambati J
Molecular therapy : the journal of the American Society of Gene Therapy 2012 Jan;20(1):101-8
Molecular therapy : the journal of the American Society of Gene Therapy 2012 Jan;20(1):101-8
Generation of retinal pigment epithelial cells from small molecules and OCT4 reprogrammed human induced pluripotent stem cells.
Krohne TU, Westenskow PD, Kurihara T, Friedlander DF, Lehmann M, Dorsey AL, Li W, Zhu S, Schultz A, Wang J, Siuzdak G, Ding S, Friedlander M
Stem cells translational medicine 2012 Feb;1(2):96-109
Stem cells translational medicine 2012 Feb;1(2):96-109
Equivalence of conventionally-derived and parthenote-derived human embryonic stem cells.
Harness JV, Turovets NA, Seiler MJ, Nistor G, Altun G, Agapova LS, Ferguson D, Laurent LC, Loring JF, Keirstead HS
PloS one 2011 Jan 7;6(1):e14499
PloS one 2011 Jan 7;6(1):e14499
Enhanced retinal pigment epithelium regeneration after injury in MRL/MpJ mice.
Xia H, Krebs MP, Kaushal S, Scott EW
Experimental eye research 2011 Dec;93(6):862-72
Experimental eye research 2011 Dec;93(6):862-72
Mitochondria impairment correlates with increased sensitivity of aging RPE cells to oxidative stress.
He Y, Ge J, Burke JM, Myers RL, Dong ZZ, Tombran-Tink J
Journal of ocular biology, diseases, and informatics 2010 Sep;3(3):92-108
Journal of ocular biology, diseases, and informatics 2010 Sep;3(3):92-108
Altered expression of retinal molecular markers in the canine RPE65 model of Leber congenital amaurosis.
Hernández M, Pearce-Kelling SE, Rodriguez FD, Aguirre GD, Vecino E
Investigative ophthalmology & visual science 2010 Dec;51(12):6793-802
Investigative ophthalmology & visual science 2010 Dec;51(12):6793-802
A protocol for the culture and differentiation of highly polarized human retinal pigment epithelial cells.
Sonoda S, Spee C, Barron E, Ryan SJ, Kannan R, Hinton DR
Nature protocols 2009;4(5):662-73
Nature protocols 2009;4(5):662-73
Steroids do not prevent photoreceptor degeneration in the light-exposed T4R rhodopsin mutant dog retina irrespective of AP-1 inhibition.
Gu D, Beltran WA, Pearce-Kelling S, Li Z, Acland GM, Aguirre GD
Investigative ophthalmology & visual science 2009 Jul;50(7):3482-94
Investigative ophthalmology & visual science 2009 Jul;50(7):3482-94
The in vitro and in vivo behaviour of retinal pigment epithelial cells cultured on ultrathin collagen membranes.
Thumann G, Viethen A, Gaebler A, Walter P, Kaempf S, Johnen S, Salz AK
Biomaterials 2009 Jan;30(3):287-94
Biomaterials 2009 Jan;30(3):287-94
CD133 progenitor cells from the bone marrow contribute to retinal pigment epithelium repair.
Harris JR, Fisher R, Jorgensen M, Kaushal S, Scott EW
Stem cells (Dayton, Ohio) 2009 Feb;27(2):457-66
Stem cells (Dayton, Ohio) 2009 Feb;27(2):457-66
Retinal Astrocytes respond to IL-17 differently than Retinal Pigment Epithelial cells.
Ke Y, Jiang G, Sun D, Kaplan HJ, Shao H
Journal of leukocyte biology 2009 Dec;86(6):1377-84
Journal of leukocyte biology 2009 Dec;86(6):1377-84
Induction of retinal pigment epithelium properties in ciliary margin progenitor cells.
Vossmerbaeumer U, Kuehl S, Kern S, Kluter H, Jonas JB, Bieback K
Clinical & experimental ophthalmology 2008 May;36(4):358-66
Clinical & experimental ophthalmology 2008 May;36(4):358-66
Iron regulates L-cystine uptake and glutathione levels in lens epithelial and retinal pigment epithelial cells by its effect on cytosolic aconitase.
Lall MM, Ferrell J, Nagar S, Fleisher LN, McGahan MC
Investigative ophthalmology & visual science 2008 Jan;49(1):310-9
Investigative ophthalmology & visual science 2008 Jan;49(1):310-9
Reactivation of uveitogenic T cells by retinal astrocytes derived from experimental autoimmune uveitis-prone B10RIII mice.
Jiang G, Ke Y, Sun D, Han G, Kaplan HJ, Shao H
Investigative ophthalmology & visual science 2008 Jan;49(1):282-9
Investigative ophthalmology & visual science 2008 Jan;49(1):282-9
Characterization of a spontaneous mouse retinal pigment epithelial cell line B6-RPE07.
Chen M, Muckersie E, Robertson M, Fraczek M, Forrester JV, Xu H
Investigative ophthalmology & visual science 2008 Aug;49(8):3699-706
Investigative ophthalmology & visual science 2008 Aug;49(8):3699-706
Novel organotypic culture model of adult mammalian neurosensory retina in co-culture with retinal pigment epithelium.
Kaempf S, Walter P, Salz AK, Thumann G
Journal of neuroscience methods 2008 Aug 15;173(1):47-58
Journal of neuroscience methods 2008 Aug 15;173(1):47-58
Aging enhances pressure-induced arterial superoxide formation.
Jacobson A, Yan C, Gao Q, Rincon-Skinner T, Rivera A, Edwards J, Huang A, Kaley G, Sun D
American journal of physiology. Heart and circulatory physiology 2007 Sep;293(3):H1344-50
American journal of physiology. Heart and circulatory physiology 2007 Sep;293(3):H1344-50
Human cone photoreceptor dependence on RPE65 isomerase.
Jacobson SG, Aleman TS, Cideciyan AV, Heon E, Golczak M, Beltran WA, Sumaroka A, Schwartz SB, Roman AJ, Windsor EA, Wilson JM, Aguirre GD, Stone EM, Palczewski K
Proceedings of the National Academy of Sciences of the United States of America 2007 Sep 18;104(38):15123-8
Proceedings of the National Academy of Sciences of the United States of America 2007 Sep 18;104(38):15123-8
Genetics and phenotypes of RPE65 mutations in inherited retinal degeneration.
Thompson DA, Gyürüs P, Fleischer LL, Bingham EL, McHenry CL, Apfelstedt-Sylla E, Zrenner E, Lorenz B, Richards JE, Jacobson SG, Sieving PA, Gal A
Investigative ophthalmology & visual science 2000 Dec;41(13):4293-9
Investigative ophthalmology & visual science 2000 Dec;41(13):4293-9
Rpe65 is necessary for production of 11-cis-vitamin A in the retinal visual cycle.
Redmond TM, Yu S, Lee E, Bok D, Hamasaki D, Chen N, Goletz P, Ma JX, Crouch RK, Pfeifer K
Nature genetics 1998 Dec;20(4):344-51
Nature genetics 1998 Dec;20(4):344-51
Molecular characterization of the human gene encoding an abundant 61 kDa protein specific to the retinal pigment epithelium.
Nicoletti A, Wong DJ, Kawase K, Gibson LH, Yang-Feng TL, Richards JE, Thompson DA
Human molecular genetics 1995 Apr;4(4):641-9
Human molecular genetics 1995 Apr;4(4):641-9
Molecular cloning and expression of RPE65, a novel retinal pigment epithelium-specific microsomal protein that is post-transcriptionally regulated in vitro.
Hamel CP, Tsilou E, Pfeffer BA, Hooks JJ, Detrick B, Redmond TM
The Journal of biological chemistry 1993 Jul 25;268(21):15751-7
The Journal of biological chemistry 1993 Jul 25;268(21):15751-7
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Supportive validation
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- antibodies-online (provider)
- Main image
- Experimental details
- WB
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- ICC
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- IHC
