PA5-25343

antibody from Invitrogen Antibodies

Targeting: DEPDC1 DEP.8, DEPDC1A, FLJ20354, SDP35

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-25343

Provider

Invitrogen Antibodies

Product name

DEPDC1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DEPDC1 in U251 cell line lysates. Samples were incubated with DEPDC1 polyclonal antibody (Product # PA5-25343). Lysates: 35 µg/lane. DEPDC1 protein (arrow).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DEPDC1 in various lysates. Samples were incubated with DEPDC1 polyclonal antibody (Product # PA5-25343) using a dilution of 1:1,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins at 30 µg per lane. Lane 1: 293T/17 whole cell lysate; Lane 2: A431 whole cell lysate. Predicted band size: 93 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of DEPDC1 in formalin fixed and paraffin embedded human testis tissue. Samples were incubated with DEPDC1 polyclonal antibody (Product # PA5-25343) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of DEPDC1 in formalin fixed and paraffin embedded human testis tissue. Samples were incubated with DEPDC1 polyclonal antibody (Product # PA5-25343) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.