GTX82932

antibody from GeneTex

Targeting: ALB

ELISA

Immunohistochemistry

Antibody data

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Validation data

Product number: GTX82932 - Provider product page
Provider: GeneTex
Proper citation: GeneTex Cat#GTX82932, RRID:AB_774261
Product name: Albumin antibody
Antibody type: Polyclonal
Reactivity: Human
Host: Rabbit
Storage: Store vial at -20¢X C prior to opening. Aliquot contents and freeze at -20¢X C or below for extended storage. Avoid cycles of freezing and thawing.

ALB protein structure - GTX82932 shown in red.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Dot Blot of Rabbit IgG protein. Antigen: control Rabbit IgG protein. Load: 250ng starting total load in a serial 1:1 dilution. Primary antibody: Rabbit IgG protein. Secondary antibody: Rabbit IgG Antibody Peroxidase Conjugated and Rabbit IgG Antibody DyLight 649 Conjugated secondary antibodies at 1:20,000 for 45 min at RT. Block buffer overnight at 4°C. Blot was dried and imaged (A)30 sec, DyLight649, (B) LiCor Odyssey Reader (700 nm), (C ) Rewetted incubated with Femtomax 110 reimaged using BioVersaDoc (for 60 sec), (D) Incubated with TMB substrate TMBM for 5 minutes and scanned, and (E) Re-wetted for chemiluminescence and imaged.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Western Blot of human serum albumin (GTX82932). Lane 1: 1:400 of FBS. Lane 2: 1:1600 of FBS. Lane 3: 1:400 of normal rabbit serum. Lane 4: 1:1600 of normal rabbit serum. Lane 5: 1:400 of normal human serum. Lane 6: 1:1600 of normal human serum. Lane 7: 1:10 of human serum albumin (10mg/mL). Lane 8: 1:40 of human serum albumin (10mg/mL). Lane 9: molecular weight. Right Blot: Secondary only incubated for control. Primary antibody: human serum albumin at 1:400 for overnight at 4°C. Secondary antibody: Biotin conjugated rabbit anti human serum albumin with 10 mg/ml BSA added to stock was diluted 1:5000 in PBST for 2hrs at RT. Dylight 649 conjugated Stretavidin 1:50000 in PBST 1 hr RT was used as a secondary reagent. Block: 1.5 % rabbit serum overnight at 4°C.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Western Blot of human serum albumin (GTX82932). Lane 1: 1:400 of FBS. Lane 2: 1:1600 of FBS. Lane 3: 1:400 of normal rabbit serum. Lane 4: 1:1600 of normal rabbit serum. Lane 5: 1:400 of normal human serum. Lane 6: 1:1600 of normal human serum. Lane 7: 1:10 of human serum albumin (10mg/mL). Lane 8: 1:40 of human serum albumin (10mg/mL). Lane 9: molecular weight. Right Blot: Secondary only incubated for control. Primary antibody: human serum albumin at 1:400 for overnight at 4¢XC. Secondary antibody: Biotin conjugated rabbit anti human serum albumin with 10 mg/ml BSA added to stock was diluted 1:5000 in PBST for 2hrs at RT. Dylight 649 conjugated Stretavidin 1:50000 in PBST 1 hr RT was used as a secondary reagent. Block: 1.5 % rabbit serum overnight at 4¢XC.