Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX78420 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX78420, RRID:AB_1080886
- Product name
- Albumin antibody (HRP)
- Antibody type
- Polyclonal
- Antigen
- Full length Human protein
- Host
- Rabbit
- Conjugate
- Horseradish Peroxidase
- Isotype
- IgG
- Vial size
- 500µg
- Concentration
- 1 mg/ml
- Storage
- Keep as concentrated solution. Store at 4°C short term. For extended storage add 50% glycerol, aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western Blot of human serum albumin (GTX78420). Lane 1: 1:400 of FBS. Lane 2: 1:1600 of FBS. Lane 3: 1:400 of normal rabbit serum. Lane 4: 1:1600 of normal rabbit serum. Lane 5: 1:400 of normal human serum. Lane 6: 1:1600 of normal human serum. Lane 7: 1:10 of human serum albumin (10mg/mL). Lane 8: 1:40 of human serum albumin (10mg/mL). Lane 9: molecular weight. Right Blot: Secondary only incubated for control. Primary antibody: human serum albumin at 1:400 for overnight at 4°C. Secondary antibody: Biotin conjugated rabbit anti human serum albumin with 10 mg/ml BSA added to stock was diluted 1:5000 in PBST for 2hrs at RT. Dylight 649 conjugated Streptavidin 1:50000 in PBST 1 hr RT was used as a secondary reagent. Block: 1.5 % rabbit serum overnight at 4°C.
- Validation comment
- WB