GTX78420

antibody from GeneTex

Targeting: ALB

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

GTX78420

Provider

GeneTex

Proper citation

GeneTex Cat#GTX78420, RRID:AB_1080886

Product name

Albumin antibody (HRP)

Antibody type

Polyclonal

Antigen

Full length Human protein

Host

Rabbit

Conjugate

Horseradish Peroxidase

Isotype

IgG

Vial size

500µg

Concentration

1 mg/ml

Storage

Keep as concentrated solution. Store at 4°C short term. For extended storage add 50% glycerol, aliquot and store at -20°C or below. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western Blot of human serum albumin (GTX78420). Lane 1: 1:400 of FBS. Lane 2: 1:1600 of FBS. Lane 3: 1:400 of normal rabbit serum. Lane 4: 1:1600 of normal rabbit serum. Lane 5: 1:400 of normal human serum. Lane 6: 1:1600 of normal human serum. Lane 7: 1:10 of human serum albumin (10mg/mL). Lane 8: 1:40 of human serum albumin (10mg/mL). Lane 9: molecular weight. Right Blot: Secondary only incubated for control. Primary antibody: human serum albumin at 1:400 for overnight at 4°C. Secondary antibody: Biotin conjugated rabbit anti human serum albumin with 10 mg/ml BSA added to stock was diluted 1:5000 in PBST for 2hrs at RT. Dylight 649 conjugated Streptavidin 1:50000 in PBST 1 hr RT was used as a secondary reagent. Block: 1.5 % rabbit serum overnight at 4°C.

Validation comment

WB