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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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- Product number
- IC1455G - Provider product page
- Provider
- R&D Systems
- Product name
- Human Serum Albumin Alexa Fluor® 488-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Serum Albumin in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- 188835
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Hepatic FcRn regulates albumin homeostasis and susceptibility to liver injury.
Pyzik M, Rath T, Kuo TT, Win S, Baker K, Hubbard JJ, Grenha R, Gandhi A, Krämer TD, Mezo AR, Taylor ZS, McDonnell K, Nienaber V, Andersen JT, Mizoguchi A, Blumberg L, Purohit S, Jones SD, Christianson G, Lencer WI, Sandlie I, Kaplowitz N, Roopenian DC, Blumberg RS
Proceedings of the National Academy of Sciences of the United States of America 2017 Apr 4;114(14):E2862-E2871
Proceedings of the National Academy of Sciences of the United States of America 2017 Apr 4;114(14):E2862-E2871
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Albumin in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Serum Albumin Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC1455G, filled histogram) or isotype control antibody (Catalog # IC003G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
