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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- NB600-868 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-868, RRID:AB_10001528
- Product name
- Mouse Monoclonal Albumin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. This antibody reacts with bovine serum albumin. The antibody recognizes a 66 kDa band corresponding to bovine serum albumin monomer. By Western blot, no cross reactivity is detected against other serum proteins. No reactivity is detected by ELISA against human serum albumin or ovalbumin.This product recognizes a 66 kDa band corresponding to an albumin monomer. No reactivity is detected by ELISA against ovalbumin.
- Reactivity
- Bovine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Bovine Serum Albumin Antibody (5F9) [NB600-868] - Lane 1: Bovine Serum Albumin. Load: 50 ng per lane. Primary antibody: Bovine Serum Albumin antibody at 1:1,000for overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:40,000 for 30 min at RT. Block: 0.75% Casein for 30 min at RT. Predicted/Observed size: 60 kDa, 60 kDa for Bovine Serum Albumin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Bovine Serum Albumin Antibody (5F9) [NB600-868] - Bovine Serum Albumin Antibody (5F9) is shown to detect BSA in bovine serum diluted 1:100 (lane 1) and in purified BSA preparations at 0.5 mg/ml (lane 2).
