Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations [0]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ABIN457981 - Provider product page
- Provider
- antibodies-online
- Proper citation
- Antibodies-Online Cat#ABIN457981, RRID:AB_10767092
- Product name
- anti-Albumin (ALB) antibody (Biotin)
- Antibody type
- Polyclonal
- Antigen
- Albumin is a stable small polypeptide with a strong antigenicity. Its molecular weight is about 69,000. It has a high mobility in electrophoresis, shows macro-heterogeneity especially under pathological con-ditions and it can bind a large number of physiological and non-physiological molecules. Albumin is isolated from human serum by sequential precipitation and purified by ion exchange chromatography and affinity chromatography. Freund?s complete adjuvant is used in the first step of the immunization procedure.
- Description
- Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 1 mL
- Concentration
- IgG protein concentration 10 mg/ml. Biotin/IgG protein molar ratio (B/P) approximately 5.3. No foreign proteins added.
- Storage
- The lyophilized immunoconjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile di stilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
No comments: Submit comment
No validations: Submit validation data