ABIN457983

antibody from antibodies-online

Targeting: ALB

ELISA (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ABIN457983

Provider

antibodies-online

Proper citation

Antibodies-Online Cat#ABIN457983, RRID:AB_10764331

Product name

anti-Albumin (ALB) antibody (HRP)

Antibody type

Polyclonal

Antigen

Albumin is a stable small polypeptide with a strong antigenicity. Its molecular weight is about 69,000. It has a high mobility in electrophoresis, shows macro-heterogeneity especially under pathological conditions and it can bind a large number of physiological and non-physiological molecules. Albumin is isolated from human serum by sequential precipitation and purified by ion exchange chromatography and affinity chromatography. Freund?s complete adjuvant is used in the first step of the immunization procedure.

Description

Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate the antibody activity to any other component of the human serum proteins. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.

Reactivity

Human

Host

Goat

Conjugate

Horseradish Peroxidase

Isotype

IgG

Vial size

1 mL

Concentration

IgG protein concentration 10 mg/ml. Enzyme/IgG protein molar ratio (E/P) is approximately 1.7. No foreign proteins added. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ = 3.2).

Storage

The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconstituted by adding 1 ml ster ile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C , not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.