16019-1-AP

antibody from Proteintech Group

Targeting: DBR1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

16019-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#16019-1-AP, RRID:AB_2230316

Product name

DBR1 antibody

Antibody type

Polyclonal

Description

KD/KO validated DBR1 antibody (Cat. #16019-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IF, IHC, WB,ELISA.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

The debranching enzyme Dbr1 regulates lariat turnover and intron splicing.

Buerer L, Clark NE, Welch A, Duan C, Taggart AJ, Townley BA, Wang J, Soemedi R, Rong S, Lin CL, Zeng Y, Katolik A, Staley JP, Damha MJ, Mosammaparast N, Fairbrother WG
Research square 2023 Jun 13;

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The Role of DBR1 as a Candidate Prognosis Biomarker in Esophageal Squamous Cell Carcinoma.

Xu X, Yang X, Liu X, Bi Y, Kong P, Wang Y, Cheng X, Xi Y
Technology in cancer research & treatment 2022 Jan-Dec;21:15330338221083105

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Inborn Errors of RNA Lariat Metabolism in Humans with Brainstem Viral Infection.

Zhang SY, Clark NE, Freije CA, Pauwels E, Taggart AJ, Okada S, Mandel H, Garcia P, Ciancanelli MJ, Biran A, Lafaille FG, Tsumura M, Cobat A, Luo J, Volpi S, Zimmer B, Sakata S, Dinis A, Ohara O, Garcia Reino EJ, Dobbs K, Hasek M, Holloway SP, McCammon K, Hussong SA, DeRosa N, Van Skike CE, Katolik A, Lorenzo L, Hyodo M, Faria E, Halwani R, Fukuhara R, Smith GA, Galvan V, Damha MJ, Al-Muhsen S, Itan Y, Boeke JD, Notarangelo LD, Studer L, Kobayashi M, Diogo L, Fairbrother WG, Abel L, Rosenberg BR, Hart PJ, Etzioni A, Casanova JL
Cell 2018 Feb 22;172(5):952-965.e18

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Identification of the specific interactors of the human lariat RNA debranching enzyme 1 protein.

Masaki S, Yoshimoto R, Kaida D, Hata A, Satoh T, Ohno M, Kataoka N
International journal of molecular sciences 2015 Feb 9;16(2):3705-21

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Inhibition of U4 snRNA in human cells causes the stable retention of polyadenylated pre-mRNA in the nucleus.

Hett A, West S
PloS one 2014;9(5):e96174

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Cell cycle regulation of microtubule interactomes: multi-layered regulation is critical for the interphase/mitosis transition.

Syred HM, Welburn J, Rappsilber J, Ohkura H
Molecular & cellular proteomics : MCP 2013 Nov;12(11):3135-47

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Inhibition of RNA lariat debranching enzyme suppresses TDP-43 toxicity in ALS disease models.

Armakola M, Higgins MJ, Figley MD, Barmada SJ, Scarborough EA, Diaz Z, Fang X, Shorter J, Krogan NJ, Finkbeiner S, Farese RV Jr, Gitler AD
Nature genetics 2012 Dec;44(12):1302-9

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

human brain tissue were subjected to SDS PAGE followed by western blot with 16019-1-AP(DBR1 antibody) at dilution of 1:500

Sample type

tissue

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using DBR1 antibody 16019-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human placenta using 16019-1-AP(DBR1 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human placenta using 16019-1-AP(DBR1 antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue