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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [2]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00051429-M03 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00051429-M03, RRID:AB_1580886
- Product name
- SNX9 monoclonal antibody (M03), clone 3C6
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant SNX9.
- Antigen sequence
MATKARVMYDFAAEPGNNELTVNEGEIITITNPDV
GGGWLEGRNIKGERGLVPTDYVEILPSDGKDQFSC
GNSVADQAFLDSLSASTAHASSSAASNNHQVGSGN
DPWSAWSASKSGNWESSEGWGAQPEGAGAQRNTNT
PNNWDTAFGHPQAYQGPATGDDDDWDEDWDGPKSS
SYFKDSESADAGGAQRGNSRASSSSMKIPLNKFPG
FAEPGTEQYLLAKQLAKPKEKIPIIVGDYGPMWVY
PTSTFDCVVADPRKGSKMYGLKSYIEYQLTPTNTN
RSVNHRYKHFDWLYERLLVKFGSAIPIPSLPDKQV
TGRFEEEFIKMRMERLQAWMTRMCRHPVISESEVF
QQFLNFRDEKEWKTGKRKAERDELAGVMIFSTMEP
EAPDLDLVEIEQKCEAVGKFTKAMDDGVKELLTVG
QEHWKRCTGPLPKEYQKIGKALQSLATVFSSSGYQ
GETDLNDAITEAGKTYEEIASLVAEQPKKDLHFLM
ECNHEYKGFLGCFPDVIGTHKGAIEKVKESDKLVA
TSKITLQDKQNMVKRVSIMSYALQAEMNHFHSNRI
YDYNSVIRLYLEQQVQFYETIAEKLRQALSRFPVM- Isotype
- IgG
- Antibody clone number
- 3C6
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of SNX9 expression in transfected 293T cell line by SNX9 monoclonal antibody (M03), clone 3C6.Lane 1: SNX9 transfected lysate (Predicted MW: 66.6 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- SNX9 monoclonal antibody (M03), clone 3C6. Western Blot analysis of SNX9 expression in human liver.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged SNX9 is 0.3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged SNX9 is 0.03 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of SNX9 transfected lysate using anti-SNX9 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with SNX9 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
