MA1-23275

antibody from Invitrogen Antibodies

Targeting: RBBP4 lin-53, NURF55, RbAp48

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Recommended by provider)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-23275

Provider

Invitrogen Antibodies

Product name

RbAp48 Monoclonal Antibody (13D10)

Antibody type

Monoclonal

Antigen

Other

Description

Recommended positive controls: HeLa nuclear extract, SH-SY-5Y, SH-SY-5Y nuclear extract.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

13D10

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Dual retinoblastoma-binding proteins with properties related to a negative regulator of ras in yeast.

Qian YW, Lee EY
The Journal of biological chemistry 1995 Oct 27;270(43):25507-13

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Dual retinoblastoma-binding proteins with properties related to a negative regulator of ras in yeast.

Qian YW, Lee EY
The Journal of biological chemistry 1995 Oct 27;270(43):25507-13

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A retinoblastoma-binding protein related to a negative regulator of Ras in yeast.

Qian YW, Wang YC, Hollingsworth RE Jr, Jones D, Ling N, Lee EY
Nature 1993 Aug 12;364(6438):648-52

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of RbAp48 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: RbAp48 Monoclonal Antibody (13D10) (Product # MA1-23275) diluted at 1:200. Red: phalloidin, a cytoskeleton marker. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of RbAp48 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with RbAp48 Monoclonal Antibody (13D10) (Product # MA1-23275) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MA1-23275 antibody immunoprecipitates RbAp48 protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract with 5 µg preimmune mouse IgG (A) or 5 µg of RbAp48 antibody (Product # MA1-23275) (B). The precipitated DNA was detected by PCR with primer set targeting to SOX9 gene locus.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of RbAp48 was performed in HeLa whole cell extract using 5 µg of RbAp48 Monoclonal Antibody (13D10) (Product # MA1-23275). Samples were transferred to a membrane and probed with RbAp48 Monoclonal Antibody (13D10) as a primary antibody and an HRP-conjugated HRP-conjugated anti mouse IgG was used as a secondary antibody.