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LearnGTX70234
antibody from GeneTex
Targeting: RBBP4
lin-53, NURF55, RbAp48
Western blot Immunocytochemistry Immunoprecipitation
Immunohistochemistry Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- GTX70234 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX70234, RRID:AB_372871
- Product name
- RbAp48 antibody [13D10]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
Submitted references Differential distribution of unmodified and phosphorylated histone deacetylase 2 in chromatin.
Differential association of products of alternative transcripts of the candidate tumor suppressor ING1 with the mSin3/HDAC1 transcriptional corepressor complex.
Stable histone deacetylase complexes distinguished by the presence of SANT domain proteins CoREST/kiaa0071 and Mta-L1.
Targeting histone deacetylase complexes via KRAB-zinc finger proteins: the PHD and bromodomains of KAP-1 form a cooperative unit that recruits a novel isoform of the Mi-2alpha subunit of NuRD.
Sun JM, Chen HY, Davie JR
The Journal of biological chemistry 2007 Nov 9;282(45):33227-36
The Journal of biological chemistry 2007 Nov 9;282(45):33227-36
Differential association of products of alternative transcripts of the candidate tumor suppressor ING1 with the mSin3/HDAC1 transcriptional corepressor complex.
Skowyra D, Zeremski M, Neznanov N, Li M, Choi Y, Uesugi M, Hauser CA, Gu W, Gudkov AV, Qin J
The Journal of biological chemistry 2001 Mar 23;276(12):8734-9
The Journal of biological chemistry 2001 Mar 23;276(12):8734-9
Stable histone deacetylase complexes distinguished by the presence of SANT domain proteins CoREST/kiaa0071 and Mta-L1.
Humphrey GW, Wang Y, Russanova VR, Hirai T, Qin J, Nakatani Y, Howard BH
The Journal of biological chemistry 2001 Mar 2;276(9):6817-24
The Journal of biological chemistry 2001 Mar 2;276(9):6817-24
Targeting histone deacetylase complexes via KRAB-zinc finger proteins: the PHD and bromodomains of KAP-1 form a cooperative unit that recruits a novel isoform of the Mi-2alpha subunit of NuRD.
Schultz DC, Friedman JR, Rauscher FJ 3rd
Genes & development 2001 Feb 15;15(4):428-43
Genes & development 2001 Feb 15;15(4):428-43
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Detection of RbAp48 in HeLa nuclear extract (GTX24105)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SH-SY-5Y whole cell and nuclear extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp48 antibody [13D10] (GTX70234) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RbAp48 antibody [13D10] detects RbAp48 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RbAp48 protein stained by RbAp48 antibody [13D10] (GTX70234) diluted at 1:200.Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RbAp48 antibody immunoprecipitates RbAp48 protein in IP experiments. IP Sample: 1000 ?g HeLa whole cell lysate/extract A. 40 £gg HeLa whole cell lysate/extract B. Control with 2.5 £gg of preimmune mouse IgG C. Immunoprecipitation of RbAp48 protein by 2.5 £gg of RbAp48 antibody (GTX70234) 10% SDS-PAGE The immunoprecipitated RbAp48 protein was detected by RbAp48 antibody (GTX70234) diluted at 1:500. EasyBlot anti-rabbit IgG (GTX221667-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RbAp48 antibody immunoprecipitates RbAp48 protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 £gg preimmune mouse IgG B. 5 £gg of RbAp48 antibody (GTX70234) The precipitated DNA was detected by PCR with primer set targeting to SOX9 gene locus.
