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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- AF3606 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Rat M-Ras/R-Ras3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human and rat M-Ras/R-Ras3 in Western blots. Does not detect recombinant human R-Ras or TC21 (R-Ras2).
- Reactivity
- Human, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O14807- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references CCL20 mediates RANK/RANKL-induced epithelial-mesenchymal transition in endometrial cancer cells.
Liu Y, Wang J, Ni T, Wang L, Wang Y, Sun X
Oncotarget 2016 May 3;7(18):25328-39
Oncotarget 2016 May 3;7(18):25328-39
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of Human and Rat M-Ras/R-Ras3 by Western Blot. Western blot shows lysates of L6 rat myoblast cell line. PVDF membrane was probed with 2 µg/mL Goat Anti-Human/Rat M-Ras/R-Ras3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3606) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human R-Ras, TC21/R-Ras2, and M-Ras/R-Ras3 (2 ng/lane) were included. A specific band for M-Ras/R-Ras3 was detected at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
