LS-C105010

antibody from LSBio

Targeting: PIK3CB PIK3C1

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C105010

Provider

LSBio

Proper citation

LifeSpan Cat#LS-C105010, RRID:AB_2165409

Product name

PIK3CB / PI3K Beta Antibody (clone 4H2) LS-C105010

Antibody type

Monoclonal

Description

Purified from ascites by Protein A

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

4H2

Storage

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to PIK3CB on formalin-fixed paraffin-embedded human lymph node (antibody concentration 3 ug/ml).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to PIK3CB on formalin-fixed paraffin-embedded human lymph node (antibody concentration 3 ug/ml).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to PIK3CB on formalin-fixed paraffin-embedded human lymph node (antibody concentration 3 ug/ml).

Proximity ligation assay

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Proximity Ligation Analysis (PLA) of protein-protein interactions between HCK and PIK3CB. Huh7 cells were stained with anti-HCK rabbit purified polyclonal 1:1200 and anti-PIK3CB mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Proximity Ligation Analysis (PLA) of protein-protein interactions between HCK and PIK3CB HeLa cells were stained with anti-HCK rabbit purified polyclonal 1:1200 and anti-PIK3CB mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Proximity Ligation Analysis (PLA) of protein-protein interactions between HCK and PIK3CB HeLa cells were stained with anti-HCK rabbit purified polyclonal 1:1200 and anti-PIK3CB mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Proximity Ligation Analysis (PLA) of protein-protein interactions between HCK and PIK3CB. Huh7 cells were stained with anti-HCK rabbit purified polyclonal 1:1200 and anti-PIK3CB mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Proximity Ligation Analysis (PLA) of protein-protein interactions between HCK and PIK3CB HeLa cells were stained with anti-HCK rabbit purified polyclonal 1:1200 and anti-PIK3CB mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Proximity Ligation Analysis (PLA) of protein-protein interactions between HCK and PIK3CB. Huh7 cells were stained with anti-HCK rabbit purified polyclonal 1:1200 and anti-PIK3CB mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection