Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP8017d - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP8017d, RRID:AB_10234407
- Product name
- PIK3CB Antibody (N-term S139)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- PIK3CB Antibody (N-term S139) (Cat. #AP8017d) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the PIK3CB antibody detected the PIK3CB protein (arrow).
- Primary Ab dilution
- 1:1000
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Western blot analysis of PIK3CB (arrow) using rabbit polyclonal PIK3CB Antibody (N-term S139) (Cat. #AP8017d). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PIK3CB gene.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- PIK3CB Antibody (N-term S139) (Cat. #AP8017d)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PIK3CB Antibody (N-term S139) for immunohistochemistry. Clinical relevance has not been evaluated.
- Primary Ab dilution
- 1:10~50