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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-22152 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-PIK3CB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: H1299, C2C12, C2C12+AEA10mM, PC-12. Predicted reactivity: Mouse (98%), Rat (97%), Chicken (91%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PIK3CB Polyclonal Antibody (Product # PA5-22152). Sample (30 µg of whole cell lysate). Lane A: H1299. 7.5% SDS PAGE. PIK3CB Polyclonal Antibody (Product # PA5-22152) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PI3K p110 beta antibody [N3C2], Internal detects PIK3CB protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract.5 % SDS-PAGE. PI3K p110 beta antibody [N3C2], Internal PIK3CB Polyclonal Antibody (Product # PA5-22152) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PIK3CB Polyclonal Antibody (Product # PA5-22152). Sample (50 µg of whole cell lysate). Lane A: C2C12. Lane B: C2C12+AEA10mM. PIK3CB Polyclonal Antibody (Product # PA5-22152) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PI3 Kinase p110 beta in methanol-fixed HeLa cells using a PI3 Kinase p110 beta polyclonal antibody (Product # PA5-22152) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of PIK3CB was performed in paraffin-embedded rat testis tissue using PIK3CB Polyclonal Antibody (Product # PA5-22152) at a dilution of 1:400.
