Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-15249 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PIK3CB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a PIK3CB polyclonal antibody (Product # PA5-15249) in MDA-MB435 cell lysates (35 µg per lane).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PIK3CB (arrow) using a PIK3CB polyclonal antibody (Product # PA5-15249) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PIK3CB gene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-PIK3CB Polyclonal Antibody (Product # PA5-15249) and a 123 kDa band corresponding to Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform was observed across tissues tested. Tissue extracts (30 µg lysate) of Mouse Thyroid (Lane 1), Mouse Stomach (Lane 2), Mouse Mammary Gland (Lane 3), Rat Mammary Gland (Lane 4), Mouse Ovary (Lane 5), Mouse Lung (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The Blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:6000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). Few uncharacterized bands were observed between 60-80 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human pancreas tissue using a PIK3CB polyclonal antibody (Product # PA5-15249), followed by HRP-conjugated secondary antibody and DAB staining.