PA5-96557

antibody from Invitrogen Antibodies

Targeting: COPB2 beta'-COP, betaprime-COP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

PA5-96557

Provider

Invitrogen Antibodies

Product name

COPB2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: AYQLAVEAES EQKWKQLAEL AISKCQFGLA QECLHHAQDY GGLLLLATAS GNANMVNKLA EGAERDGKNN VAFMSYFLQG KVDACLELLI RTGRLPEAAF LARTYLPSQV SRVVKLWREN LSKVNQKAAE SLADPTEYEN LFPGLKEAFV VEEWVKETHA DLWPAKQYPL VTPNEERNVM EEGKDFQPSR STAQQELDGK PASPTPVIVA SHTANKEEKS LLELEVDLDN LELEDIDTTD INLDEDILDD; Positive Samples: SW480, MCF7, HeLa, HepG2, Jurkat, Mouse liver, Mouse kidney, Mouse stomach; Cellular Location: COPI-coated vesicle membrane, Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Golgi apparatus membrane, Peripheral membrane protein, cytosol

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.54 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of COPB2 in extracts of various cell lines using COPB2 Polyclonal Antibody (Product # PA5-96557) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using COPB2 Polyclonal Antibody (Product # PA5-96557) diluted 1:1,000. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) diluted 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL. Exposure time: 180 s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of COPB2 was performed in HeLa cells using COPB2 Polyclonal Antibody (Product # PA5-96557) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of COPB2 was performed in NIH/3T3 cells using COPB2 Polyclonal Antibody (Product # PA5-96557) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of COPB2 was performed in PC-12 cells using COPB2 Polyclonal Antibody (Product # PA5-96557).