PA5-142780

antibody from Invitrogen Antibodies

Targeting: NMNAT3 PNAT3

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-142780

Provider

Invitrogen Antibodies

Product name

NMNAT3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Additional Information: This antibody is expected to recognize both reported isoforms (NP_835471.1; NP_001186976.1). This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:32,000.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Samples were incubated with NMNAT3 polyclonal antibody (Product # PA5-142780) using a dilution of 10 µg/mL for 1 hr followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Samples were incubated with NMNAT3 polyclonal antibody (Product # PA5-142780) using a dilution of 10 µg/mL for 1hr followed by Alexa Fluor 488 secondary antibody at a dilution of 1 µg/mL. IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.