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Western blot
ELISA
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Other assay [1]
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- Product number
- PA5-95964 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLR2D Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MAAGGSDPRA GDVEEDASQL IFPKEFETAE TLLNSEVHML LEHRKQQNES AEDEQELSEV FMKTLNYTAR FSRFKNRETI ASVRSLLLQK KLHKFELACL ANLCPETAEE SKALIPSLEG RFEDEELQQI LDDIQTKRSF QY; Positive Samples: MCF-7, SW480, SW620, HepG2, SK-HEPC, Mouse spleen; Cellular Location: Nucleus
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.24 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of POLR2D in A549 cells. Samples were incubated with POLR2D Polyclonal antibody (Product # PA5-95964).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of POLR2D was performed in A549 cells using POLR2D Polyclonal Antibody (Product # PA5-95964).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of POLR2D in 200 μg extracts of HepG2 cells. Samples were precipitated with 1 μg POLR2D Polyclonal antibody (Product # PA5-95964). Western blot was performed from the immunoprecipitate using POLR2D Polyclonal antibody (Product # PA5-95964) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of POLR2D was performed in 200 µg extracts of HepG2 cells using POLR2D Polyclonal Antibody (Product # PA5-95964). Western blot was performed from the immunoprecipitate using POLR2D Polyclonal Antibody at a dilution of 1:1000.
