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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
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- Product number
- PA5-21772 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SAP130 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: HeLa. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SAP130 Polyclonal Antibody detects SAP130 protein by western blot analysis. A. 30 µg HeLa whole cell extract.5 % SDS-PAGE. SAP130 Polyclonal Antibody (Product # PA5-21772) dilution: 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SAP130 in methanol-fixed HeLa cells using a SAP130 polyclonal antibody (Product # PA5-21772) at a 1:500 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using SAP130 (Product # PA5-21772) antibody at 1:500 dilution.
