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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB4334 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4334, RRID:AB_1578746
- Product name
- PIGK polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PIGK.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Two subunits of glycosylphosphatidylinositol transamidase, GPI8 and PIG-T, form a functionally important intermolecular disulfide bridge.
Structural requirements for the recruitment of Gaa1 into a functional glycosylphosphatidylinositol transamidase complex.
PIG-S and PIG-T, essential for GPI anchor attachment to proteins, form a complex with GAA1 and GPI8.
Ohishi K, Nagamune K, Maeda Y, Kinoshita T
The Journal of biological chemistry 2003 Apr 18;278(16):13959-67
The Journal of biological chemistry 2003 Apr 18;278(16):13959-67
Structural requirements for the recruitment of Gaa1 into a functional glycosylphosphatidylinositol transamidase complex.
Vainauskas S, Maeda Y, Kurniawan H, Kinoshita T, Menon AK
The Journal of biological chemistry 2002 Aug 23;277(34):30535-42
The Journal of biological chemistry 2002 Aug 23;277(34):30535-42
PIG-S and PIG-T, essential for GPI anchor attachment to proteins, form a complex with GAA1 and GPI8.
Ohishi K, Inoue N, Kinoshita T
The EMBO journal 2001 Aug 1;20(15):4088-98
The EMBO journal 2001 Aug 1;20(15):4088-98
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The PIGK polyclonal antibody (Cat # PAB4334) is used in Western blot to detect PIGK in 293 cell lysate .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with PIGK polyclonal antibody (Cat # PAB4334) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated . BC = breast carcinoma .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
