H00000483-M05

antibody from Abnova Corporation

Targeting: ATP1B3 CD298, FLJ29027

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000483-M05

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000483-M05, RRID:AB_509177

Product name

ATP1B3 monoclonal antibody (M05), clone 2F4

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant ATP1B3.

Antigen sequence

MTKNEKKSLNQSLAEWKLFIYNPTTGEFLGRTAKS
WGLILLFYLVFYGFLAALFSFTMWVMLQTLNDEVP
KYRDQIPSPGLMVFPKPVTALEYTFSRSDPTSYAG
YIEDLKKFLKPYTLEEQKNLTVCPDGALFEQKGPV
YVACQFPISLLQACSGMNDPDFGYSQGNPCILVKM
NRIIGLKPEGVPRIDCVSKNEDIPNVAVYPHNGMI
DLKYFPYYGKKLHVGYLQPLVAVQVSFAPNNTGKE
VTVECKIDGSANLKSQDDRDKFLGRVMFKITARA


Isotype

IgG

Antibody clone number

2F4

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

The importance of adequate fixation for immunofluorescent staining of bovine embryos.

Goossens K, Vandaele L, Wydooghe E, Thys M, Dewulf J, Peelman LJ, Van Soom A
Reproduction in domestic animals = Zuchthygiene 2011 Dec;46(6):1098-103

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ATP1B3 is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol