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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- ANP-013-25UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATP1B3 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human Jurkat T-cell leukemia cell line lysates (lanes 1 and 3) and human THP-1 monocytic leukemia cell line lysates (lanes 2 and 4): - 1, 2. Anti-ATP1B3 (extracellular) Antibody (#ANP-013), (1:200). 3, 4. Anti-ATP1B3 (extracellular) Antibody , preincubated with ATP1B3 (extracellular) Blocking Peptide (#BLP-NT013).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of ATP1B3 in rat hippocampus. Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-ATP1B3 (extracellular) Antibody (#ANP-013), followed by goat Anti-rabbit-AlexaFluor-488. A. Staining in the in rat hippocampal dentate gyrus region, revealed immunoreactivity (green) in neurons of the hilus (H, arrows) and the granule layer (G). B. Pre-incubation of the Antibody with ATP1B3 (extracellular) Blocking Peptide (BLP-NP013), suppressed staining. Cell nuclei are stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of ATP1B3 in live intact human THP-1 monocytic leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-ATP1B3 (extracellular) Antibody (#ANP-013), (2.5 µg) + goat- Anti-rabbit-FITC.
