Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-56987 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CCDC115 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PREAGLRRRK GPTKTPEPES SEAPQDPLNW FGILVPHSLR QAQASFRDGL QLAADIASLQ NRIDWGRSQL RGLQEKLKQL EP Highest antigen sequence identity to the following orthologs: Mouse - 83%, Rat - 84%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.4 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Swine Host Protein Coiled-Coil Domain-Containing 115 (CCDC115) Interacts with Classical Swine Fever Virus Structural Glycoprotein E2 during Virus Replication.
Vuono EA, Ramirez-Medina E, Berggren K, Rai A, Pruitt S, Silva E, Velazquez-Salinas L, Gladue DP, Borca MV
Viruses 2020 Mar 31;12(4)
Viruses 2020 Mar 31;12(4)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of CCDC115 in human colon tissue shows strong cytoplasmic positivity in glandular cells. Samples were probed using a CCDC115 Polyclonal Antibody (Product # PA5-56987).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Interaction between CSFV E2 and CCDC115 in CSFV-infected cells demonstrated by proximity ligation assay (PLA). Images were visualized at 40X magnification. SK6 cells ( A ) or primary swine macrophages ( B ) that were either mock infected (Mock) or infected for 24 h with CSFV parental BICv or recombinant E2DeltaCCDC115v (MOI = 10) as described in Materials and Methods. Panel B (on the right), quantification of the decrease in the E2-CCDC115 interaction in swine macrophages infected with either BICv or E2DeltaCCDC115v, error bars represent the average and standard deviation from from the counting of three independent counts of 100 cells. *** p -value < 0.0008.