MA1-10152

antibody from Invitrogen Antibodies

Targeting: PRNP AltPrP, CD230, CJD, GSS, PRIP, PRP

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-10152

Provider

Invitrogen Antibodies

Product name

PrP Monoclonal Antibody (EM-20)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This antibody recognizes human prion protein (PrP). Diglycosylated form of PrP has ~ 40 kDa, monoglycosylated form ~ 30 kDa, and nonglycosylated form ~ 19-21 kDa. This antibody is suitable for discrimination between normal cellular prion protein (PrPc) and its conformationally changed form (PrPSc) prion protein. Western Blot: non-reducing conditions are essential.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

EM-20

Vial size

100 µg

Concentration

1 mg/mL

Storage

4° C, do not freeze

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blotting analysis of Creutzfeld-Jakob disease (CJD) negative (lane 1, 2) and CJD positive (lane 3, 4) human brain material using anti-PrP monoclonal antibody (Product # MA1-10152). CJD positive patient has proteinase K resistent prion protein. Lane 1, 4: Samples with proteinase K treatment Lane 2, 3: Samples without proteinase K treatment

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blotting analysis of Creutzfeld-Jakob disease (CJD) negative (lane 1, 2) and CJD positive (lane 3, 4) human brain material using anti-PrP (clone EM-20) Monoclonal antibody (Product # MA1-10152). CJD positive patient has proteinase K resistent prion protein. Lane 1, 4: Samples with proteinase K treatment. Lane 2, 3: Samples without proteinase K treatment.