14-9230-82

antibody from Invitrogen Antibodies
Targeting: PRNP AltPrP, CD230, CJD, GSS, PRIP, PRP
Provider product page for 14-9230-82
Western blot
Immunoprecipitation
Flow cytometry
Other assay

Antibody data

Product number
14-9230-82 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD230 (PrP) Monoclonal Antibody (4D5), eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: CD230 (prion protein; PrP^C) is a 35 kD glycoprotein, attached to the cell surface via a GPI-anchor. While the function of CD230 is unclear, prion protein has been the focus of research based on its involvement in transmissible spongiform encephalopathies (TSE), including Creutzfeld-Jakob disease in humans. CD230 undergoes a conformational change in which the cellular PrP^C form is converted to the scrapie PrP^Sc form. This conversion is essential for the infectiousness of prion-based diseases. CD230 is predominantly expressed on the surface of lymphocytes and monocytes, with weak expression on granulocytes and erythrocytes. In platelets, CD230 is expressed primarily intracellularly, and is upregulated to the surface upon activation. Applications Reported: This 4D5 antibody has been reported for use in flow cytometric analysis and immunoprecipitation. The 4D5 antibody has also been found useful for immunoblotting, recognizing a protein of approximately 35 kD under non-reducing conditions. Applications Tested: The 4D5 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
4D5
Vial size
100 µg
Concentration
0.5 mg/mL
Storage
4° C
PRNP protein structure - 14-9230-82 shown in red.