MA5-32202

antibody from Invitrogen Antibodies

Targeting: PRNP AltPrP, CD230, CJD, GSS, PRIP, PRP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-32202

Provider

Invitrogen Antibodies

Product name

CD230 (PrP) Recombinant Rabbit Monoclonal Antibody (SC57-05)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

SC57-05

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Extracellular Protein Aggregates Colocalization and Neuronal Dystrophy in Comorbid Alzheimer's and Creutzfeldt-Jakob Disease: A Micromorphological Pilot Study on 20 Brains.

Jankovska N, Olejar T, Matej R
International journal of molecular sciences 2021 Feb 20;22(4)

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Extracellular Prion Protein Aggregates in Nine Gerstmann-Sträussler-Scheinker Syndrome Subjects with Mutation P102L: A Micromorphological Study and Comparison with Literature Data.

Jankovska N, Matej R, Olejar T
International journal of molecular sciences 2021 Dec 10;22(24)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CD230 (PrP) in different lysates using a Monoclonal antibody (Product #MA5-32202) at a dilution of 1:1,000. Positive control: Lane 1: Rat brain, Lane 2: Mouse brain.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of CD230 (PrP) in SHG-44 cells using a CD230 (PrP) Monoclonal antibody (Product # MA5-32202) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of CD230 (PrP) in N2A cells using a CD230 (PrP) Monoclonal antibody (Product # MA5-32202) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of CD230 (PrP) in SH-SY-5Y cells using a CD230 (PrP) Monoclonal antibody (Product # MA5-32202) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometric analysis of CD230 (PrP) in SH-SY-5Y cells using a CD230 (PrP) Monoclonal Antibody (Product # MA5-32202) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Illustration--Parallel observation of ( a , b ) multicentric and ( c , d ) solitary kuru-like plaques with centrally bright cores visualized using immunohistochemistry and immunofluorescence. ( a , c ) Primary antibody in immunohistochemical images: PrP (mouse monoclonal antibody). The secondary antibody was conjugated with horseradish peroxidase staining DAB. The original magnification was 100x. ( b , d ) Primary antibodies in immunofluorescent images: PrP (rabbit recombinant monoclonal antibody, green color) + AT8 (mouse monoclonal antibody, red color). The secondary antibody was conjugated with either Alexa Fluor (r) 488 (anti-rabbit IgG, green) or Alexa Fluor (r) 568 (anti-mouse IgG, red). Scale bars indicate 25 um in ( a , b ) and 10 um in ( c , d ).