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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- PA5-27313 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PrP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: IMR32, Neuro2A, NIH-3T3, C2C12. Predicted reactivity: Mouse (89%), Rat (89%), Dog (88%), Pig (90%), Rabbit (91%), Sheep (90%), Rhesus Monkey (95%), Chimpanzee (98%), Bovine (87%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.36 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PRP antibody detects PRNP protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg NIH-3T3 whole cell lysate/extract. C. 30 µg C2C12 whole cell lysate/extract.12 % SDS-PAGE. PRP antibody PrP Polyclonal Antibody (Product # PA5-27313) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PrP Polyclonal Antibody (Product # PA5-27313). Sample (30 µg of whole cell lysate). Lane A:IMR32. 12% SDS PAGE. PrP Polyclonal Antibody (Product # PA5-27313) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PRP antibody detects PRNP protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg NIH-3T3 whole cell lysate/extract. C. 30 µg C2C12 whole cell lysate/extract.12 % SDS-PAGE. PRP antibody PrP Polyclonal Antibody (Product # PA5-27313) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PRP antibody detects PRP protein at nucleus on mouse middle brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse middle brain. PRP antibody (Product # PA5-27313) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
