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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-31293 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDE10A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HCT116, mouse brain, rat brain, Myc-DDK-tagged PDE10A-transfected 293T. Predicted reactivity: Mouse (98%), Rat (98%), Zebrafish (84%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.97 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Phosphodiesterase 10A IgG: A novel biomarker of paraneoplastic neurologic autoimmunity.
Zekeridou A, Kryzer T, Guo Y, Hassan A, Lennon V, Lucchinetti CF, Pittock S, McKeon A
Neurology 2019 Aug 20;93(8):e815-e822
Neurology 2019 Aug 20;93(8):e815-e822
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PDE10A in methanol-fixed HeLa cells using a PDE10A polyclonal antibody (Product # PA5-31293) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PDE10A (Product # PA5-31293) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Immunohistochemistry of renal cell carcinoma (patient 4) (A, B) Phosphodiesterase 10A (PDE10A) immunohistochemistry in normal control human kidney tissue. The high power image (B) indicates the collecting tubular epithelium with marked expression of PDE10A. The kidney carcinoma tissue of patient 4 shows foci of variable PDE10A immunoreactivity: moderate expression (C, D) or scant PDE10A expression (E, F) in different parts of the tumor. Scale bars in A, C, and E = 100 mum. Scale bars in B, D, and F = 20 mum.
