21214-1-AP

antibody from Proteintech Group

Targeting: PLOD2 LH2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

21214-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#21214-1-AP, RRID:AB_10733347

Product name

PLOD2-Specific antibody

Antibody type

Polyclonal

Description

KD/KO validated PLOD2-Specific antibody (Cat. #21214-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Decrease of lysyl hydroxylase 2 activity causes abnormal collagen molecular phenotypes, defective mineralization and compromised mechanical properties of bone.

Saito T, Terajima M, Taga Y, Hayashi F, Oshima S, Kasamatsu A, Okubo Y, Ito C, Toshimori K, Sunohara M, Tanzawa H, Uzawa K, Yamauchi M
Bone 2022 Jan;154:116242

---

Lysyl hydroxylase 2 deficiency promotes filopodia formation and fibroblast migration.

Nozaki R, Kasamatsu A, Moss J, Uzawa K
Biochemical and biophysical research communications 2022 Jan 8;587:146-152

---

PLOD2-driven IL-6/STAT3 signaling promotes the invasion and metastasis of oral squamous cell carcinoma via activation of integrin β1.

Saito K, Mitsui A, Sumardika IW, Yokoyama Y, Sakaguchi M, Kondo E
International journal of oncology 2021 Jun;58(6)

---

Lysyl hydroxylase 2-induced collagen cross-link switching promotes metastasis in head and neck squamous cell carcinomas.

Sato K, Parag-Sharma K, Terajima M, Musicant AM, Murphy RM, Ramsey MR, Hibi H, Yamauchi M, Amelio AL
Neoplasia (New York, N.Y.) 2021 Jun;23(6):594-606

---

TGFβ-1 Induced Cross-Linking of the Extracellular Matrix of Primary Human Dermal Fibroblasts.

Semkova ME, Hsuan JJ
International journal of molecular sciences 2021 Jan 20;22(3)

---

Adipose-derived stromal cells improve functional recovery after spinal cord injury through TGF-β1/Smad3/PLOD2 pathway activation.

Li F, Liu H, Zhang K, Xiao DJ, Wang C, Wang YS
Aging 2021 Jan 20;13(3):4370-4387

---

PLOD2 increases resistance of gastric cancer cells to 5-fluorouracil by upregulating BCRP and inhibiting apoptosis.

Wang X, Guo J, Dai M, Wang T, Yang T, Xiao X, Tang Q, Zhang L, Jia L
Journal of Cancer 2020;11(12):3467-3475

---

LTBP1 promotes esophageal squamous cell carcinoma progression through epithelial-mesenchymal transition and cancer-associated fibroblasts transformation.

Cai R, Wang P, Zhao X, Lu X, Deng R, Wang X, Su Z, Hong C, Lin J
Journal of translational medicine 2020 Mar 26;18(1):139

---

PLOD2 promotes aerobic glycolysis and cell progression in colorectal cancer by upregulating HK2.

Du W, Liu N, Zhang Y, Liu X, Yang Y, Chen W, He Y
Biochemistry and cell biology = Biochimie et biologie cellulaire 2020 Jun;98(3):386-395

---

Aberrant Collagen Cross-linking in Human Oral Squamous Cell Carcinoma.

Saito T, Uzawa K, Terajima M, Shiiba M, Amelio AL, Tanzawa H, Yamauchi M
Journal of dental research 2019 May;98(5):517-525

---

Deficiency of lysyl hydroxylase 2 in mice causes systemic endoplasmic reticulum stress leading to early embryonic lethality.

Kasamatsu A, Uzawa K, Hayashi F, Kita A, Okubo Y, Saito T, Kimura Y, Miyamoto I, Oka N, Shiiba M, Ito C, Toshimori K, Miki T, Yamauchi M, Tanzawa H
Biochemical and biophysical research communications 2019 May 7;512(3):486-491

---

Cyclophilin B control of lysine post-translational modifications of skin type I collagen.

Terajima M, Taga Y, Cabral WA, Liu Y, Nagasawa M, Sumida N, Kayashima Y, Chandrasekaran P, Han L, Maeda N, Perdivara I, Hattori S, Marini JC, Yamauchi M
PLoS genetics 2019 Jun;15(6):e1008196

---

Role of Glycosyltransferase 25 Domain 1 in Type I Collagen Glycosylation and Molecular Phenotypes.

Terajima M, Taga Y, Sricholpech M, Kayashima Y, Sumida N, Maeda N, Hattori S, Yamauchi M
Biochemistry 2019 Dec 17;58(50):5040-5051

---

A Feedback Loop between Hypoxia and Matrix Stress Relaxation Increases Oxygen-Axis Migration and Metastasis in Sarcoma.

Lewis DM, Pruitt H, Jain N, Ciccaglione M, McCaffery JM, Xia Z, Weber K, Eisinger-Mathason TSK, Gerecht S
Cancer research 2019 Apr 15;79(8):1981-1995

---

Hypoxia and TGF-β1 induced PLOD2 expression improve the migration and invasion of cervical cancer cells by promoting epithelial-to-mesenchymal transition (EMT) and focal adhesion formation.

Xu F, Zhang J, Hu G, Liu L, Liang W
Cancer cell international 2017;17:54

---

A scalable lysyl hydroxylase 2 expression system and luciferase-based enzymatic activity assay.

Guo HF, Cho EJ, Devkota AK, Chen Y, Russell W, Phillips GN Jr, Yamauchi M, Dalby KN, Kurie JM
Archives of biochemistry and biophysics 2017 Mar 15;618:45-51

---

Hypoxia-induced PLOD2 promotes proliferation, migration and invasion via PI3K/Akt signaling in glioma.

Song Y, Zheng S, Wang J, Long H, Fang L, Wang G, Li Z, Que T, Liu Y, Li Y, Zhang X, Fang W, Qi S
Oncotarget 2017 Jun 27;8(26):41947-41962

---

FKBP65-dependent peptidyl-prolyl isomerase activity potentiates the lysyl hydroxylase 2-driven collagen cross-link switch.

Chen Y, Terajima M, Banerjee P, Guo H, Liu X, Yu J, Yamauchi M, Kurie JM
Scientific reports 2017 Apr 5;7:46021

---

Procollagen-lysine 2-oxoglutarate 5-dioxygenase 2 promotes hypoxia-induced glioma migration and invasion.

Xu Y, Zhang L, Wei Y, Zhang X, Xu R, Han M, Huang B, Chen A, Li W, Zhang Q, Li G, Wang J, Zhao P, Li X
Oncotarget 2017 Apr 4;8(14):23401-23413

---

Loss of Type I Collagen Telopeptide Lysyl Hydroxylation Causes Musculoskeletal Abnormalities in a Zebrafish Model of Bruck Syndrome.

Gistelinck C, Witten PE, Huysseune A, Symoens S, Malfait F, Larionova D, Simoens P, Dierick M, Van Hoorebeke L, De Paepe A, Kwon RY, Weis M, Eyre DR, Willaert A, Coucke PJ
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2016 Nov;31(11):1930-1942

---

Glyoxalase 1-knockdown in human aortic endothelial cells - effect on the proteome and endothelial function estimates.

Stratmann B, Engelbrecht B, Espelage BC, Klusmeier N, Tiemann J, Gawlowski T, Mattern Y, Eisenacher M, Meyer HE, Rabbani N, Thornalley PJ, Tschoepe D, Poschmann G, Stühler K
Scientific reports 2016 Nov 29;6:37737

---

Regulation of the collagen cross-linking enzymes LOXL2 and PLOD2 by tumor-suppressive microRNA-26a/b in renal cell carcinoma.

Kurozumi A, Kato M, Goto Y, Matsushita R, Nishikawa R, Okato A, Fukumoto I, Ichikawa T, Seki N
International journal of oncology 2016 May;48(5):1837-46

---

Hypoxic stellate cells of pancreatic cancer stroma regulate extracellular matrix fiber organization and cancer cell motility.

Sada M, Ohuchida K, Horioka K, Okumura T, Moriyama T, Miyasaka Y, Ohtsuka T, Mizumoto K, Oda Y, Nakamura M
Cancer letters 2016 Mar 28;372(2):210-8

---

Absence of the ER Cation Channel TMEM38B/TRIC-B Disrupts Intracellular Calcium Homeostasis and Dysregulates Collagen Synthesis in Recessive Osteogenesis Imperfecta.

Cabral WA, Ishikawa M, Garten M, Makareeva EN, Sargent BM, Weis M, Barnes AM, Webb EA, Shaw NJ, Ala-Kokko L, Lacbawan FL, Högler W, Leikin S, Blank PS, Zimmerberg J, Eyre DR, Yamada Y, Marini JC
PLoS genetics 2016 Jul;12(7):e1006156

---

Tumour-suppressive miRNA-26a-5p and miR-26b-5p inhibit cell aggressiveness by regulating PLOD2 in bladder cancer.

Miyamoto K, Seki N, Matsushita R, Yonemori M, Yoshino H, Nakagawa M, Enokida H
British journal of cancer 2016 Jul 26;115(3):354-63

---

Intratumoral oxygen gradients mediate sarcoma cell invasion.

Lewis DM, Park KM, Tang V, Xu Y, Pak K, Eisinger-Mathason TS, Simon MC, Gerecht S
Proceedings of the National Academy of Sciences of the United States of America 2016 Aug 16;113(33):9292-7

---

Lysyl hydroxylase 2 induces a collagen cross-link switch in tumor stroma.

Chen Y, Terajima M, Yang Y, Sun L, Ahn YH, Pankova D, Puperi DS, Watanabe T, Kim MP, Blackmon SH, Rodriguez J, Liu H, Behrens C, Wistuba II, Minelli R, Scott KL, Sanchez-Adams J, Guilak F, Pati D, Thilaganathan N, Burns AR, Creighton CJ, Martinez ED, Zal T, Grande-Allen KJ, Yamauchi M, Kurie JM
The Journal of clinical investigation 2015 Mar 2;125(3):1147-62

---

Gene expression analysis of murine and human osteoarthritis synovium reveals elevation of transforming growth factor β-responsive genes in osteoarthritis-related fibrosis.

Remst DF, Blom AB, Vitters EL, Bank RA, van den Berg WB, Blaney Davidson EN, van der Kraan PM
Arthritis & rheumatology (Hoboken, N.J.) 2014 Mar;66(3):647-56

---

TGF-ß induces Lysyl hydroxylase 2b in human synovial osteoarthritic fibroblasts through ALK5 signaling.

Remst DF, Blaney Davidson EN, Vitters EL, Bank RA, van den Berg WB, van der Kraan PM
Cell and tissue research 2014 Jan;355(1):163-71

---

Hypoxia-dependent modification of collagen networks promotes sarcoma metastasis.

Eisinger-Mathason TS, Zhang M, Qiu Q, Skuli N, Nakazawa MS, Karakasheva T, Mucaj V, Shay JE, Stangenberg L, Sadri N, Puré E, Yoon SS, Kirsch DG, Simon MC
Cancer discovery 2013 Oct;3(10):1190-205

---

Osteoarthritis-related fibrosis is associated with both elevated pyridinoline cross-link formation and lysyl hydroxylase 2b expression.

Remst DF, Blaney Davidson EN, Vitters EL, Blom AB, Stoop R, Snabel JM, Bank RA, van den Berg WB, van der Kraan PM
Osteoarthritis and cartilage 2013 Jan;21(1):157-64

---

PLOD2 induced under hypoxia is a novel prognostic factor for hepatocellular carcinoma after curative resection.

Noda T, Yamamoto H, Takemasa I, Yamada D, Uemura M, Wada H, Kobayashi S, Marubashi S, Eguchi H, Tanemura M, Umeshita K, Doki Y, Mori M, Nagano H
Liver international : official journal of the International Association for the Study of the Liver 2012 Jan;32(1):110-8

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

DU 145 cells were subjected to SDS PAGE followed by western blot with 21214-1-AP(PLOD2 antibody) at dilution of 1:300

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using PLOD2 antibody 21214-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-PLOD2 (IP:21214-1-AP, 4ug; Detection:21214-1-AP 1:500) with mouse testis tissue lysate 5000ug.

Sample type

tissue

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human liver cancer using 21214-1-AP(PLOD2 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human liver cancer using 21214-1-AP(PLOD2 antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue