Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-38625 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RSK3 Monoclonal Antibody (3C4C8)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- This antibody has been tested in indirect ELISA.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3C4C8
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RSK3 using RSK3 Monoclonal Antibody (Product # MA5-38625) in HeLa (1), A431 (2), HEK293 (3), Jurkat (4), HepG2 (5), MCF-7 (6), NIH/3T3 (7) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RSK3 using RSK3 Monoclonal Antibody (Product # MA5-38625) in HEK293 (1) and RPS6KA2 (AA: 415-734)-human IgG-Fc transfected HEK293 (2) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RSK3 using RSK3 Monoclonal Antibody (Product # MA5-38625) in HeLa (1), A431 (2), HEK293 (3), Jurkat (4), HepG2 (5), MCF-7 (6), NIH/3T3 (7) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis using RSK3 Monoclonal Antibody (Product # MA5-38625) against human RPS6KA2 (AA: 415-734) recombinant protein. (Expected MW is 62 kDa)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RSK3 using RSK3 Monoclonal Antibody (Product # MA5-38625) in HEK293 (1) and RPS6KA2 (AA: 415-734)-human IgG-Fc transfected HEK293 (2) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of RSK3 in A431 cells using RSK3 Monoclonal Antibody (Product # MA5-38625) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Product # 35503)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of RSK3 in HeLa cells using RSK3 Monoclonal Antibody (Product # MA5-38625) (green) and negative control (red).