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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AF893 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Phospho-RSK3 (S218) Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human and mouse RSK3 when phosphorylated at S218. Reactivity with other RSK isoforms when phosphorylated at corresponding sites is unknown.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C, as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 6 months, -20 to -70 °C under sterile conditions after opening.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Mouse Phospho-RSK3 (S218) by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (Catalog # 120-HD) for for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse Phospho-RSK3 (S218) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF893), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-RSK3 (S218) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
