PA1-745

antibody from Invitrogen Antibodies

Targeting: CNR1 CANN6, CB-R, CB1, CB1A, CB1K5, CNR

Flow cytometry

Antibody data

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Validation data

Product number: PA1-745 - Provider product page
Provider: Invitrogen Antibodies
Product name: Cannabinoid Receptor 1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Other
Description: PA1-745 detects cannabinoid receptor 1 (CB1) from human, rat, monkey, mouse, chicken, salamander and goldfish tissues. This antibody detects a few non-specific bands in NIH-3T3 and HT-29 cell lysates. PA1-745 has been successfully used in Western blot, FACS, immunohistochemistry, and immunocytochemistry procedures. By Western blot, this antibody detects a ~60 kDa protein representing CB1 and less intense bands of ~23 kDa, ~72 kDa, and ~180 kDa from rat brain homogenate. Immunocytochemical staining of CB1 in living or fixed cultured hippocampal neurons with PA1-745 results in intense, punctate staining along processes, with little staining over cell bodies. PA1-745 antigen is a fusion protein containing the first 77 amino acid residues of the rat CB1 receptor.
Reactivity: Human, Mouse, Rat, Chicken/Avian
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1.0 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

CNR1 protein structure - PA1-745 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4. Expression of the novel lipases in the mouse nervous system. DAGLalpha (a) and DAGbeta (b) are expressed in neurofilament-positive axonal tracts (c) in the embryonic spinal cord (day 10). DAGLbeta is expressed in the embryonic retinal ganglion fiber layer and optic nerve at day 14 (d). Note the lack of expression of DAGLalpha in the optic (e) and anterior commissures (f) in the adult. This contrasts with strong staining for the CB1 receptor in these tracts (g and h). DAGLalpha (i) and DAGLbeta (j) expression in the adult cerebellum is restricted to the deep cerebellar nuclei and synaptic fields, including the Purkinje cell dendrites (k). Bar: (a-c) ~130 mum; (d) ~220 mum; (e-j) ~500 mum; (k) ~20 mum.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 1 Figure Validation of antibodies for PLAs. Expression of heteromers in striatal sections using two different anti-CB 1 R antibodies. (A) Striatal neurons showing PLA label for CB 1 R/GPR55Hets using a rabbit antibody raised against the N-terminus of the CB 1 R and with a rabbit anti-GPR55 antibody. (B) Striatal neurons showing PLA label for CB 1 R/GPR55Hets detected using a rabbit anti-GPR55 antibody and a goat antibody raised against the C-terminal domain of the CB 1 R. Nuclei are labeled with TO-PRO-3. Scale bar 50 mum.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 3 Figure Expression of CB 1 R-GPR55 heteromeric complexes in different subtypes of striatal projection neurons and interneurons. Triple immunofluorescent stains were performed to detect CB 1 R/GPR55Hets (by PLA), BDA-labeled neurons, and striatal interneurons positive for either ChAT, PV, CR, or nNOS. PV + neurons are by far the striatal neuron subtype containing the highest amount of heteromers.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 (a-b) CB 1 and CB 2 protein expression determined by flow cytometry. Changes were measured as increase in mean fluorescence intensity ( MFI ) of gated lymphocytes/monocytes (a) CB 1 and CB 2 in whole blood (left panel). (b) CB 1 and CB 2 in PBMC (right panel). See, text for significance levels. Results shown are representative of five independent experiments. Control = rabbit IgG.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 2 Immunohistochemistry for CB 1 and CB 2 receptors, FAAH and MAGL in human colonic tissue. Morphology of normal human colon, stained with H&E (A-C). General views of transmural sections through the colon (A, D, G, J, M). High-magnification photomicrographs of the colonic epithelium and lamina propria (B, E, H, K, N), smooth muscle and myenteric plexus (C, F, I, L, O). Abbreviations : C, crypt; CSM, circular smooth muscle; LP, lamina propria; LSM, longitudinal smooth muscle; M, mucosa; MM, muscularis mucosae, MP, myenteric plexus; SM, submucosa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 3 Protein expression of cannabinoid CB1 (B,D) and CB2 receptor (A,C) in the endocrine pancreas. Immunohistochemistry and densitometric analysis of CB1 (F) and CB2 (E) protein expression in pancreatic sections from 12-week-old mice showed a decrease in both CB1 and CB2 receptors in the islets of Langerhans from CB2xP mice compared to wild-type mice (n = 5 mice each genotype). Student's t-test: *P < 0.05. Images are representative of several slices. Scale bars are included in each image.