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ImmunocytochemistryAntibody data
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- Antigen structure
- References [28]
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- Immunocytochemistry [1]
- Other assay [6]
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- Product number
- PA1-743 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cannabinoid Receptor 1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- PA1-743 detects cannabinoid receptor 1 (CB1) from human and rat tissues as well as transfected rat CB1. PA1-743 has been successfully used in Western blot and immunocytochemistry procedures. By Western blot, this antibody detects an ~52 kDa protein representing CB1 from rat brain homogenate. Immunocytochemical staining of CB1 in AtT20 cells transfected with the rat CB1 gene with PA1-743 yields a pattern consistent with plasma membrane staining. PA1-743 antigen is a fusion protein containing the first 99 amino acid residues of human CB1.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Modulation of Neurolipid Signaling and Specific Lipid Species in the Triple Transgenic Mouse Model of Alzheimer's Disease.
Mechanism of Diuresis and Natriuresis by Cannabinoids: Evidence for Inhibition of Na(+)-K(+)-ATPase in Mouse Kidney Thick Ascending Limb Tubules.
Upregulation of CB(2) receptors in reactive astrocytes in canine degenerative myelopathy, a disease model of amyotrophic lateral sclerosis.
Neonatal Treatment with a Pegylated Leptin Antagonist Induces Sexually Dimorphic Effects on Neurones and Glial Cells, and on Markers of Synaptic Plasticity in the Developing Rat Hippocampal Formation.
Overactive cannabinoid 1 receptor in podocytes drives type 2 diabetic nephropathy.
Cannabinoid CB1 receptor is expressed in chromophobe renal cell carcinoma and renal oncocytoma.
A Role for the Cannabinoid 1 Receptor in Neuronal Differentiation of Adult Spinal Cord Progenitors in vitro is Revealed through Pharmacological Inhibition and Genetic Deletion.
Reversal of liver fibrosis by the antagonism of endocannabinoid CB1 receptor in a rat model of CCl(4)-induced advanced cirrhosis.
Expression and localization of cannabinoid receptors in human immature oocytes and unfertilized metaphase-II oocytes.
Genetic and pharmacologic inactivation of cannabinoid CB1 receptor inhibits angiogenesis.
Effects of chronic bhang (cannabis) administration on the reproductive system of male mice.
Effect of capacitation on the endocannabinoid system of mouse sperm.
Spinal cord injuries induce changes in CB1 cannabinoid receptor and C-C chemokine expression in brain areas underlying circuitry of chronic pain conditions.
Regulation of subthalamic neuron activity by endocannabinoids.
CB1 and CB2 cannabinoid receptor expression during development and in epileptogenic developmental pathologies.
The CB(2) cannabinoid receptor regulates human sperm cell motility.
Cannabinoid CB₁ receptor is downregulated in clear cell renal cell carcinoma.
Levels of endocannabinoids and palmitoylethanolamide and their pharmacological manipulation in chronic granulomatous inflammation in rats.
Involvement of the endocannabinoid system in periodontal healing.
Activated endocannabinoid system in coronary artery disease and antiinflammatory effects of cannabinoid 1 receptor blockade on macrophages.
Opposing control of cannabinoid receptor stimulation on amyloid-beta-induced reactive gliosis: in vitro and in vivo evidence.
Cannabinoid receptors in human astroglial tumors.
Differential expression of cannabinoid receptors in the human colon: cannabinoids promote epithelial wound healing.
Delta9-tetrahydrocannabinol selectively acts on CB1 receptors in specific regions of dorsal vagal complex to inhibit emesis in ferrets.
Cannabinoid CB2 receptors and fatty acid amide hydrolase are selectively overexpressed in neuritic plaque-associated glia in Alzheimer's disease brains.
Cannabinoid CB2 receptors and fatty acid amide hydrolase are selectively overexpressed in neuritic plaque-associated glia in Alzheimer's disease brains.
Cannabinoids acting on CB1 receptors decrease contractile performance in human atrial muscle.
Cannabinoids inhibit N- and P/Q-type calcium channels in cultured rat hippocampal neurons.
González de San Román E, Llorente-Ovejero A, Martínez-Gardeazabal J, Moreno-Rodríguez M, Giménez-Llort L, Manuel I, Rodríguez-Puertas R
International journal of molecular sciences 2021 Nov 12;22(22)
International journal of molecular sciences 2021 Nov 12;22(22)
Mechanism of Diuresis and Natriuresis by Cannabinoids: Evidence for Inhibition of Na(+)-K(+)-ATPase in Mouse Kidney Thick Ascending Limb Tubules.
Ritter JK, Ahmad A, Mummalaneni S, Daneva Z, Dempsey SK, Li N, Li PL, Lyall V
The Journal of pharmacology and experimental therapeutics 2021 Jan;376(1):1-11
The Journal of pharmacology and experimental therapeutics 2021 Jan;376(1):1-11
Upregulation of CB(2) receptors in reactive astrocytes in canine degenerative myelopathy, a disease model of amyotrophic lateral sclerosis.
Fernández-Trapero M, Espejo-Porras F, Rodríguez-Cueto C, Coates JR, Pérez-Díaz C, de Lago E, Fernández-Ruiz J
Disease models & mechanisms 2017 May 1;10(5):551-558
Disease models & mechanisms 2017 May 1;10(5):551-558
Neonatal Treatment with a Pegylated Leptin Antagonist Induces Sexually Dimorphic Effects on Neurones and Glial Cells, and on Markers of Synaptic Plasticity in the Developing Rat Hippocampal Formation.
López-Gallardo M, Antón-Fernández A, Llorente R, Mela V, Llorente-Berzal A, Prada C, Viveros MP
Journal of neuroendocrinology 2015 Aug;27(8):658-69
Journal of neuroendocrinology 2015 Aug;27(8):658-69
Overactive cannabinoid 1 receptor in podocytes drives type 2 diabetic nephropathy.
Jourdan T, Szanda G, Rosenberg AZ, Tam J, Earley BJ, Godlewski G, Cinar R, Liu Z, Liu J, Ju C, Pacher P, Kunos G
Proceedings of the National Academy of Sciences of the United States of America 2014 Dec 16;111(50):E5420-8
Proceedings of the National Academy of Sciences of the United States of America 2014 Dec 16;111(50):E5420-8
Cannabinoid CB1 receptor is expressed in chromophobe renal cell carcinoma and renal oncocytoma.
Larrinaga G, Sanz B, Blanco L, Perez I, Candenas ML, Pinto FM, Irazusta A, Gil J, López JI
Clinical biochemistry 2013 May;46(7-8):638-41
Clinical biochemistry 2013 May;46(7-8):638-41
A Role for the Cannabinoid 1 Receptor in Neuronal Differentiation of Adult Spinal Cord Progenitors in vitro is Revealed through Pharmacological Inhibition and Genetic Deletion.
Sideris A, Bekker T, Chan WS, Montoya-Gacharna JV, Blanck TJ, Recio-Pinto E
Frontiers in neuroscience 2012;6:4
Frontiers in neuroscience 2012;6:4
Reversal of liver fibrosis by the antagonism of endocannabinoid CB1 receptor in a rat model of CCl(4)-induced advanced cirrhosis.
Giannone FA, Baldassarre M, Domenicali M, Zaccherini G, Trevisani F, Bernardi M, Caraceni P
Laboratory investigation; a journal of technical methods and pathology 2012 Mar;92(3):384-95
Laboratory investigation; a journal of technical methods and pathology 2012 Mar;92(3):384-95
Expression and localization of cannabinoid receptors in human immature oocytes and unfertilized metaphase-II oocytes.
Peralta L, Agirregoitia E, Mendoza R, Expósito A, Casis L, Matorras R, Agirregoitia N
Reproductive biomedicine online 2011 Sep;23(3):372-9
Reproductive biomedicine online 2011 Sep;23(3):372-9
Genetic and pharmacologic inactivation of cannabinoid CB1 receptor inhibits angiogenesis.
Pisanti S, Picardi P, Prota L, Proto MC, Laezza C, McGuire PG, Morbidelli L, Gazzerro P, Ziche M, Das A, Bifulco M
Blood 2011 May 19;117(20):5541-50
Blood 2011 May 19;117(20):5541-50
Effects of chronic bhang (cannabis) administration on the reproductive system of male mice.
Banerjee A, Singh A, Srivastava P, Turner H, Krishna A
Birth defects research. Part B, Developmental and reproductive toxicology 2011 Jun;92(3):195-205
Birth defects research. Part B, Developmental and reproductive toxicology 2011 Jun;92(3):195-205
Effect of capacitation on the endocannabinoid system of mouse sperm.
Catanzaro G, Battista N, Rossi G, Di Tommaso M, Pucci M, Pirazzi V, Cecconi S, Maccarrone M
Molecular and cellular endocrinology 2011 Aug 22;343(1-2):88-92
Molecular and cellular endocrinology 2011 Aug 22;343(1-2):88-92
Spinal cord injuries induce changes in CB1 cannabinoid receptor and C-C chemokine expression in brain areas underlying circuitry of chronic pain conditions.
Knerlich-Lukoschus F, Noack M, von der Ropp-Brenner B, Lucius R, Mehdorn HM, Held-Feindt J
Journal of neurotrauma 2011 Apr;28(4):619-34
Journal of neurotrauma 2011 Apr;28(4):619-34
Regulation of subthalamic neuron activity by endocannabinoids.
Morera-Herreras T, Ruiz-Ortega JA, Taupignon A, Baufreton J, Manuel I, Rodriguez-Puertas R, Ugedo L
Synapse (New York, N.Y.) 2010 Sep;64(9):682-98
Synapse (New York, N.Y.) 2010 Sep;64(9):682-98
CB1 and CB2 cannabinoid receptor expression during development and in epileptogenic developmental pathologies.
Zurolo E, Iyer AM, Spliet WG, Van Rijen PC, Troost D, Gorter JA, Aronica E
Neuroscience 2010 Sep 29;170(1):28-41
Neuroscience 2010 Sep 29;170(1):28-41
The CB(2) cannabinoid receptor regulates human sperm cell motility.
Agirregoitia E, Carracedo A, Subirán N, Valdivia A, Agirregoitia N, Peralta L, Velasco G, Irazusta J
Fertility and sterility 2010 Mar 15;93(5):1378-87
Fertility and sterility 2010 Mar 15;93(5):1378-87
Cannabinoid CB₁ receptor is downregulated in clear cell renal cell carcinoma.
Larrinaga G, Sanz B, Pérez I, Blanco L, Cándenas ML, Pinto FM, Gil J, López JI
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2010 Dec;58(12):1129-34
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2010 Dec;58(12):1129-34
Levels of endocannabinoids and palmitoylethanolamide and their pharmacological manipulation in chronic granulomatous inflammation in rats.
Endocannabinoid Research Group, De Filippis D, D'Amico A, Cipriano M, Petrosino S, Orlando P, Di Marzo V, Iuvone T
Pharmacological research 2010 Apr;61(4):321-8
Pharmacological research 2010 Apr;61(4):321-8
Involvement of the endocannabinoid system in periodontal healing.
Kozono S, Matsuyama T, Biwasa KK, Kawahara K, Nakajima Y, Yoshimoto T, Yonamine Y, Kadomatsu H, Tancharoen S, Hashiguchi T, Noguchi K, Maruyama I
Biochemical and biophysical research communications 2010 Apr 16;394(4):928-33
Biochemical and biophysical research communications 2010 Apr 16;394(4):928-33
Activated endocannabinoid system in coronary artery disease and antiinflammatory effects of cannabinoid 1 receptor blockade on macrophages.
Sugamura K, Sugiyama S, Nozaki T, Matsuzawa Y, Izumiya Y, Miyata K, Nakayama M, Kaikita K, Obata T, Takeya M, Ogawa H
Circulation 2009 Jan 6;119(1):28-36
Circulation 2009 Jan 6;119(1):28-36
Opposing control of cannabinoid receptor stimulation on amyloid-beta-induced reactive gliosis: in vitro and in vivo evidence.
Esposito G, Iuvone T, Savani C, Scuderi C, De Filippis D, Papa M, Di Marzo V, Steardo L
The Journal of pharmacology and experimental therapeutics 2007 Sep;322(3):1144-52
The Journal of pharmacology and experimental therapeutics 2007 Sep;322(3):1144-52
Cannabinoid receptors in human astroglial tumors.
Held-Feindt J, Dörner L, Sahan G, Mehdorn HM, Mentlein R
Journal of neurochemistry 2006 Aug;98(3):886-93
Journal of neurochemistry 2006 Aug;98(3):886-93
Differential expression of cannabinoid receptors in the human colon: cannabinoids promote epithelial wound healing.
Wright K, Rooney N, Feeney M, Tate J, Robertson D, Welham M, Ward S
Gastroenterology 2005 Aug;129(2):437-53
Gastroenterology 2005 Aug;129(2):437-53
Delta9-tetrahydrocannabinol selectively acts on CB1 receptors in specific regions of dorsal vagal complex to inhibit emesis in ferrets.
Van Sickle MD, Oland LD, Mackie K, Davison JS, Sharkey KA
American journal of physiology. Gastrointestinal and liver physiology 2003 Sep;285(3):G566-76
American journal of physiology. Gastrointestinal and liver physiology 2003 Sep;285(3):G566-76
Cannabinoid CB2 receptors and fatty acid amide hydrolase are selectively overexpressed in neuritic plaque-associated glia in Alzheimer's disease brains.
Benito C, Núñez E, Tolón RM, Carrier EJ, Rábano A, Hillard CJ, Romero J
The Journal of neuroscience : the official journal of the Society for Neuroscience 2003 Dec 3;23(35):11136-41
The Journal of neuroscience : the official journal of the Society for Neuroscience 2003 Dec 3;23(35):11136-41
Cannabinoid CB2 receptors and fatty acid amide hydrolase are selectively overexpressed in neuritic plaque-associated glia in Alzheimer's disease brains.
Benito C, Núñez E, Tolón RM, Carrier EJ, Rábano A, Hillard CJ, Romero J
The Journal of neuroscience : the official journal of the Society for Neuroscience 2003 Dec 3;23(35):11136-41
The Journal of neuroscience : the official journal of the Society for Neuroscience 2003 Dec 3;23(35):11136-41
Cannabinoids acting on CB1 receptors decrease contractile performance in human atrial muscle.
Bonz A, Laser M, Küllmer S, Kniesch S, Babin-Ebell J, Popp V, Ertl G, Wagner JA
Journal of cardiovascular pharmacology 2003 Apr;41(4):657-64
Journal of cardiovascular pharmacology 2003 Apr;41(4):657-64
Cannabinoids inhibit N- and P/Q-type calcium channels in cultured rat hippocampal neurons.
Twitchell W, Brown S, Mackie K
Journal of neurophysiology 1997 Jul;78(1):43-50
Journal of neurophysiology 1997 Jul;78(1):43-50
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunlocalization of rat CB1 transfected into AtT20 cells using Product # PA1-743.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3. Status of CB 1 receptors in spinal samples of DM-affected dogs. Gene expression for the CB 1 receptor ( CNR1 ) and NAPE-PLD , DAGL , FAAH and MAGL measured by qPCR (A), and representative microphotographs for CB 1 receptor immunostaining using DAB (C) and its quantification in the grey matter in the ventral horn (B), in the spinal cord samples (for qPCR) or T7-T10 sections (for immunostaining) of DM-affected and age-matched control dogs. Values are expressed as means+-s.e.m. for 7-8 animals per group. Data were analysed using the unpaired Student's t -test. Scale bar: 150 um.
